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首页> 外文期刊>Advances in Forestry Science >Micropropagation of Barleria prionitis L. var. dicantha: an ethnomedicinal plant
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Micropropagation of Barleria prionitis L. var. dicantha: an ethnomedicinal plant

机译：微粉变菌Barleria trichanthum var。棘皮植物：人种植物

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Barleria prionitis var. dicantha Blatt & Hallb is an important endemic, ethno medicinal plant of Rajasthan, commonly known as Vajaradanti. It is especially well known for its antidontalgic properties. It is a rich source of glycosides, steroids, tannins and flavonoids. Natural habitat loss due to urbanization, high habitat specificity and exploitation for medicinal values places considerable pressure on native population of this endemic endangered plant. There is a need for development of non-conventional methods for propagation and conservation of B. prionitis var. dicantha . The present study was undertaken with the aim to set up a protocol for in vitro propagation of this medicinally important plant. For this, nodal segments were obtained from in vitro raised seedling on half strength MS medium supplemented with 2.88 μM GA 3 . For axillary bud break, nodal segments were inoculated on different concentrations of BA, Kn or TDZ supplemented MS medium. Maximum (75.5%) bud break was obtained on MS medium supplemented with 8.88 μM BA + additives with an average of 3.82±0.13 shoots explant -1 and 1.74±0.08 cm shoot length. The elongated shoots were excised from mother plant and further multiplied on MS medium supplemented with 4.44 μM BA. For rooting in vitro raised shoots were cultured on MS medium supplemented with different concentrations IBA or IAA. The maximum rooting was 75.5% obtained on 2.46 μM IBA with 4±0.13 roots explant -1 and 3.44±0.04 cm root length. The in vitro raised plantlets were successfully hardened and acclimatized in polybags at shade house.
机译：重症细菌性pr虫病dicantha Blatt＆Hallb是拉贾斯坦邦的一种重要地方病，民族药用植物，通常被称为Vajaradanti。它以其抗牙本质特性而闻名。它是糖苷，类固醇，单宁和类黄酮的丰富来源。由于城市化，高栖息地特异性和对药用价值的开发而导致的自然栖息地丧失，给这种特有濒危植物的本地种群带来了巨大压力。需要开发非常规方法来繁殖和保存B. prionitis var。迪坎塔。进行本研究的目的是建立该药用重要植物的体外繁殖方案。为此，在补充了2.88μMGA 3的半强度MS培养基上从体外育苗中获得节段。对于腋芽破裂，将节段接种在不同浓度的补充BA，Kn或TDZ的MS培养基上。在补充了8.88μMBA +添加剂的MS培养基上获得了最大（75.5％）的芽断裂，外植体-1的平均芽长为3.82±0.13，芽长为1.74±0.08 cm。从母株上切下细长的芽，并在补充有4.44μMBA的MS培养基上进一步繁殖。为了生根，将体外生长的芽在补充有不同浓度IBA或IAA的MS培养基上培养。在2.46μMIBA上获得的最大生根为75.5％，根植体-1为4±0.13，根长为3.44±0.04 cm。体外饲养的小苗已成功地硬化，并在阴凉处的塑料袋中适应了环境。

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