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首页> 外文期刊>ACS Omega >Zein Microneedles for Transcutaneous Vaccine Delivery: Fabrication, Characterization, and in Vivo Evaluation Using Ovalbumin as the Model Antigen
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Zein Microneedles for Transcutaneous Vaccine Delivery: Fabrication, Characterization, and in Vivo Evaluation Using Ovalbumin as the Model Antigen

机译：用于透皮疫苗递送的玉米蛋白微针：制备，表征和体内评价使用卵清蛋白作为模型抗原。

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摘要

Transcutaneous antigen administration provides an alternative to invasive syringe injections. The objective of this study was to investigate the feasibility of fabrication and antigen delivery using microneedles made from corn protein, zein. Micromolding technique was used to cast cone-shaped zein microneedles (ZMNs). The insertion of ZMNs and the delivery of the model antigen, ovalbumin (OVA), into the skin was confirmed by histological examination and confocal microscopy. In addition, a significantly (p < 0.05) lower bacterial skin penetration was observed after ZMN application compared with hypodermic syringe application. OVA coated on ZMNs was stable after storage under ambient and refrigerator conditions. Transcutaneous immunization studies showed significantly (p < 0.001) greater antibody titers (total IgG, IgG1, and IgG2a) after the application of OVA-coated ZMNs and OVA intradermal injection compared with the control group. Taken together, antigen-coated ZMNs can be developed for transcutaneous vaccine delivery.
机译：经皮抗原施用提供了有创注射器注射的替代方法。这项研究的目的是研究使用玉米蛋白玉米蛋白制成的微针制造和抗原递送的可行性。使用微成型技术铸造锥形玉米蛋白微针（ZMNs）。通过组织学检查和共聚焦显微镜证实了ZMNs的插入和模型抗原卵清蛋白（OVA）的皮肤传递。另外，与皮下注射器施用相比，ZMN施用后观察到细菌皮肤渗透显着降低（p ＜0.05）。在环境和冰箱条件下储存后，涂在ZMN上的OVA稳定。经皮免疫研究显示，与对照组相比，在施用OVA包被的ZMN和OVA皮内注射后，抗体效价（IgG，IgG1和IgG2a总量）显着提高（p <0.001）。综上所述，可以开发抗原包被的ZMN，用于经皮疫苗递送。

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