Streamlined Preparation of Immobilized Candida antarctica Lipase B

Karim Engelmark Cassimjee; Peter Hendil-Forssell; Alexey Volkov; Anne Krog; Jostein Malmo; Trond Erik V. Aune; Wolfgang Knecht; Iain R. Miskelly; Thomas S. Moody; Maria Svedendahl Humble

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Streamlined Preparation of Immobilized Candida antarctica Lipase B

机译：固定化南极假丝酵母脂肪酶B的简化制备

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Candida antarctica lipase B (CalB) was efficiently expressed (6.2 g L~(–1)) in Escherichia coli by utilizing an N-terminal tag cassette and the XylS/Pm expression system in a fed-batch bioreactor; subsequent direct binding to EziG from crude extracts resulted in an immobilized catalyst with superior activity to Novozym 435.

机译：利用N末端标签盒和XylS / Pm表达系统在饲料中有效地表达了南极念珠菌脂肪酶B（CalB）（6.2 g L〜（–1））。分批生物反应器;随后直接与粗提物中的EziG结合，产生了固定化的催化剂，其活性优于Novozym 435。

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《ACS Omega》 |2017年第12期|共4页
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Karim Engelmark Cassimjee; Peter Hendil-Forssell; Alexey Volkov; Anne Krog; Jostein Malmo; Trond Erik V. Aune; Wolfgang Knecht; Iain R. Miskelly; Thomas S. Moody; Maria Svedendahl Humble;
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1. Streamlined Preparation of Immobilized Candida antarctica Lipase B [J] . Karim Engelmark Cassimjee, Peter Hendil-Forssell, Alexey Volkov, ACS Omega . 2017,第12期

机译：固定化南极假丝酵母脂肪酶B的简化制备
2. Evaluation of the performance of differently immobilized recombinant lipase B from Candida antarctica preparations for the synthesis of pharmacological derivatives in organic media [J] . Manoel Evelin A., Robert Julia M., Pinto Martina C. C., RSC Advances . 2016,第5期

机译：评价不同固定的重组脂肪酶B从Candida抗野菌的性能进行有机培养基中药衍生物合成的制剂
3. Kinetic resolution of (R/S)-propranolol (1-isopropylamino-3-(1-naphtoxy)-2-propanolol) catalyzed by immobilized preparations of Candida antarctica lipase B (CAL-B) [J] . Oveimar Barbosa, Cesar Ariza, Claudia Ortiz, New biotechnology . 2010,第6期

机译：固定化南极假丝酵母脂肪酶B（CAL-B）催化的（R / S）-普萘洛尔（1-异丙氨基-3-（1-萘氧基）-2-丙醇）的动力学拆分
4. PRODUCTION, IMMOBILIZATION AND SYNTHESIS OF PHARMACOLOGICAL DERIVATIVES OF LIPASE B FROM CANDIDA ANTARCTICA IN PICHIA PASTORIS [C] . Julia de M. Robert, Evelin A. Manoel, Martina C. C. Pinto, Microbial engineering . 2018

机译：Pichia Pastoris中南极假丝酵母脂肪酶B的产生，固定化和合成
5. Strep-tag II fusion technology for the immobilization of lipase B from Candida antarctica [D] . Johar, Sumreet Singh. 2016

机译：STREP标签II融合技术，用于从Candida南极洲固定脂肪酶B.
6. Streamlined Preparation of Immobilized Candida antarctica Lipase B [O] . Karim Engelmark Cassimjee, *, Peter Hendil-Forssell, 2017

机译：固定化南极假丝酵母脂肪酶B的简化制备
7. Streamlined Preparation of Immobilized Candida antarctica Lipase B [O] . Karim Engelmark Cassimjee, Peter Hendil-Forssell, Alexey Volkov, 2017

机译：流线型固定化南极假丝酵母脂肪酶B的制备

Streamlined Preparation of Immobilized Candida antarctica Lipase B

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