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首页> 外文期刊>Acta Societatis Botanicorum Poloniae >Ultrastructural and antioxidative changes in lupine embryo axes in response to salt stress
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Ultrastructural and antioxidative changes in lupine embryo axes in response to salt stress

机译：盐胁迫响应羽扇豆胚轴的超微结构和抗氧化变化

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Embryo axes of lupine (Lupinus luteus L. ‘Mister’) were subjected to 0.1 M NaCl salt stress for 24 and 48 h. The ultrastructure modification and adjustment of antioxidant enzymes activities and izoenzymes profiles were observed. In cells of lupine embryo axes grown for 48 hours in medium with 0.1 M NaCl mitochondria took the forked shape and bulges of the outer mitochondrial membranes appeared. Moreover, the inflating and swelling of rough endoplasmic reticulum (RER) lumen and fragmentation of RER were noticed. The level of H2O2 was higher in salt treated embryo axes after 24 hours and increase of thiobarbituric acid reactive substances was observed after both 24 and 48 h of salt treatment. Native gel electrophoresis showed increased intensities of bands for catalase isozymes in response to salt stress, whereas activity of catalase was higher only in embryo axes grown for 48 h in control conditions. Appearance of two new isoforms of ascorbate peroxidase was observed after 48 h only under control condition, however increased activities were stated for both control and salt-stress condition after 48 h. No changes in isozymes pattern for superoxide dismutase were observed, but significant decrease in superoxide dismutase activity was noticed in relation to time and salt stress. Possible role of these enzymes in salt stress tolerance is discussed. The 0.1 M salt stress is regarded as a middle stress for lupine embryo axes and the efficiency of stress prevention mechanisms is proposed.
机译：羽扇豆（Lupinus luteus L.“ Mister”）的胚轴受到0.1 M NaCl盐胁迫持续24和48 h。观察了抗氧化酶活性和同工酶谱的超微结构修饰和调节。羽扇豆胚轴的细胞在含0.1 M NaCl线粒体的培养基中生长48小时，呈叉状，并出现线粒体外膜凸起。此外，还注意到粗糙的内质网（RER）管腔膨胀和肿胀以及RER破裂。盐处理后的胚轴中​​的H2O2水平在24小时后更高，并且在盐处理后24和48 h均观察到硫代巴比妥酸反应性物质的增加。天然凝胶电泳显示过氧化氢酶同工酶的条带强度增加，以响应盐胁迫，而过氧化氢酶的活性仅在对照条件下生长48小时的胚轴中较高。仅在对照条件下48小时后，观察到两种新的抗坏血酸过氧化物酶同工型的出现，但是在48 h后，对照条件和盐胁迫条件下活性均增加。没有观察到超氧化物歧化酶同工酶模式的变化，但是注意到与时间和盐胁迫有关的超氧化物歧化酶活性显着降低。讨论了这些酶在盐胁迫耐受性中的可能作用。 0.1 M盐胁迫被认为是羽扇豆胚胎轴的中间应力，并提出了应力预防机制的有效性。

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