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首页> 外文期刊>Acta Societatis Botanicorum Poloniae >Terpenoids from a multiple shoot culture of Telekia speciosa
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Terpenoids from a multiple shoot culture of Telekia speciosa

机译：Telekia speciosa多次芽培养物中的萜类化合物

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Multiple shoots of Telekia speciosa were cultivated on MS medium containing 4.44 μM BAP and 0.54 μM NAA, solidified with agar. After eight weeks of culture the shoots were harvested and extracted with methanol. From the methanol extract one pseudoguaianolide – 2,3-dihydroaromaticin and three thymol derivatives: 8-hydroxy-9,10-diisobutyryloxythymol, 10-isobutyryloxy-8,9-epoxythymyl isobutyrate and 10-(2-methylbutyryloxy)-8,9-epoxythymyl isobutyrate were isolated as major secondary metabolites. Moreover, the shoots produced megastigmane and monoterpene glucosides, which were isolated for the first time from the species. The content of 2,3-dihydroaromaticin in the shoot culture was similar to that found in the intact plant, whereas yields of the three thymol derivatives were higher from multiple shoots than from the plants grown in the open field.
机译：Telekia speciosa的多芽在含有4.44μMBAP和0.54μMNAA的MS培养基上培养，并用琼脂固化。培养八周后，收获芽并用甲醇萃取。从甲醇中提取一种假瓜亚诺利德– 2,3-二氢芳香族化合物和三种百里酚衍生物：8-羟基-9,10-二异丁酰氧基百里酚，10-异丁酰氧基-8,9-环氧胸苷异丁酸酯和10-（2-甲基丁酰氧基）-8,9-异丁酸环氧胸苷被分离为主要的次要代谢产物。此外，新芽还产生了巨头烯和单萜糖苷，这是首次从该物种中分离出来。枝条培养物中2，3-二氢芳族素的含量与完整植物中的含量相似，而多重枝条中三种百里酚衍生物的产量高于空田中生长的植物。

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