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Maintenance of male sterile germplasm in Brassica rapa by in vitro propagation

机译:体外繁殖维持小白菜雄性不育种质

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An efficient tissue culture system for plant regeneration, from mature cut branches, was established to maintain male sterile material in Brassica rapa L. The new-growth immature pods from the cut branches were used as explantsresults in callus initiation (37 calli from 25 explants) and shoot formation (17 shoots from 75 explants) than flower buds and branch stems. Auxin [2,4-dichlorophenoxyacetic acid (2,4-D), 2 to 5 mg l-1] and cytokinin [6-benzylaminopurine (BA), 2 to 4 mg l-1] were essential in callus and shoot formation, respectively. Callus initiation and shoot regeneration capacities were genotype dependent. The regenerated plants were male sterile and were used in breeding programs.;
机译:建立了一种有效的组织培养系统,用于从成熟的切花分支再生植物,以维持甘蓝型油菜雄性不育材料。切花分支的新生长未成熟豆荚用作愈伤组织起始的外植体结果(25个外植体中的37个愈伤组织)和芽的形成(来自75个外植体的17个芽)要比花蕾和树枝梗高。生长素[2,4-二氯苯氧基乙酸(2,4-D),2至5 mg l-1]和细胞分裂素[6-苄基氨基嘌呤(BA),2至4 mg l-1]在愈伤组织和芽形成中是必不可少的,分别。愈伤组织起始和芽再生能力是基因型依赖性的。再生的植物是雄性不育的,并用于育种程序。

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