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首页> 外文期刊>African Journal of Biotechnology >Plant regeneration studies of Jatropha curcas using induced embryogenic callus from cotyledon explants
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Plant regeneration studies of Jatropha curcas using induced embryogenic callus from cotyledon explants

机译：利用子叶外植体诱导的胚性愈伤组织进行麻疯树的植物再生研究

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摘要

A plant regeneration system for?Jatropha curcas, a biofuel- producing plant, was established from the induced embryogenic callus. Cotyledon explants cultured on single auxin media were able to form callus. However, only the callus induced from cotyledon explants using Murashige and Skoog (MS) medium containing 0.8 mg l-1dicamba was embryogenic. The somatic embryos were suitable to be proliferated using phytohormone- free woody plant medium (WPM). Two somatic embryo regeneration methods were studied and the direct somatic embryos plant regeneration method using the media containing 0.3 mg l-1?6-benzylaminopurine (BAP) and 0.4 mg l-1??gibberellic acid (GA3) was more effective, 83.3 and 73.33% plant regeneration, respectively. The other method that needed to mature the somatic embryos prior to plant regeneration from the phytohormone- free MS medium was not preferred, less than 40% plant regeneration. In conclusion, an efficient plant regeneration system for?J. curcas?was established through somatic embryogenesis. ??Keywords:?????Jatropha curcas?, cotyledons, callus , somatic embryogenesis ,? plant regeneration.
机译：从诱导的胚性愈伤组织中建立了一种用于生产麻疯树的麻疯树的植物再生系统。在单一生长素培养基上培养的子叶外植体能够形成愈伤组织。但是，只有使用含有0.8 mg -1二麦草畏的Murashige和Skoog（MS）培养基从子叶外植体中诱导的愈伤组织才具有胚发生性。体细胞胚适合使用不含植物激素的木本植物培养基（WPM）进行增殖。研究了两种体细胞胚再生方法，使用含0.3 mg l-1？6-苄基氨基嘌呤（BAP）和0.4 mgl-1β？赤霉素（GA3）的培养基的直接体胚植物再生方法更有效，分别为83.3和83.3。分别有73.33％的植物再生。在从无植物激素的MS培养基中再生植物之前，需要成熟体细胞胚的另一种方法不是优选的，少于40％的植物再生。综上所述，一种有效的植物再生系统？姜黄素是通过体细胞胚发生而建立的。关键词：麻疯树，子叶，愈伤组织，体细胞胚发生，植物再生。

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