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Sexing bovine pre-implantation embryos using the polymerase chain reaction: A model for human embryo sexing

机译:使用聚合酶链反应对牛植入前胚胎进行性别鉴定:人类胚胎性别鉴定的模型

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The paper aims to present a bovine model for human embryo sexing. Cows were super-ovulated,artificially inseminated and embryos were recovered 7 days later. Embryo biopsy was performed; DNAwas extracted from blastomeres and amplified using bovine-specific and bovine-Y-chromosomespecificprimers, followed by agarose gel electrophoresis. Embryos were then transferred tosynchronized recipients and PCR predicted sex was compared to the actual sex of the newborn calves.Results showed a 39% pregnancy rate and a 95.65% accuracy of the sexing method. The sexing methodcan also be applied to human embryos, using different primers, designed for human DNA.
机译:本文旨在提出一种用于人类胚胎性别鉴定的牛模型。将母牛超排卵,人工授精,并在7天后恢复胚胎。进行胚胎活检;从卵裂球中提取DNA,并使用牛特异性和牛Y染色体特异性引物进行扩增,然后进行琼脂糖凝胶电泳。然后将胚胎转移到同步的受者中,并将PCR预测的性别与新生犊牛的实际性别进行比较,结果显示性别分娩方法的妊娠率为39%,准确率为95.65%。也可以使用为人类DNA设计的不同引物,将性别鉴定方法应用于人类胚胎。

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