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首页> 外文期刊>African Journal of Biotechnology >The study of genetic diversity in some Iranian accessions of Hyoscyamus sp. using amplified fragment length polymorphism (AFLP) and retrotransposon/AFLP markers
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The study of genetic diversity in some Iranian accessions of Hyoscyamus sp. using amplified fragment length polymorphism (AFLP) and retrotransposon/AFLP markers

机译：伊朗部分Hyoscyamus sp。的遗传多样性研究。使用扩增片段长度多态性（AFLP）和反转录转座子/ AFLP标记

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Hyoscyamus?sp.?is well known as a natural source of two main tropan alkaloids including hyoscyamine and scopolamine. The environmental conditions make a very wide diversity of this herb in Iran. This study was conducted to evaluate the genetic diversity within a set of 45 Iranian accessions?of Hyoscyamus?sp. usingamplified fragment length polymorphism?and retro/AFLP markers. 18 primer combinations of?AFLP markers and?five retro/AFLP primer combinations were also used to estimate genetic diversity among accessions. Analysis of banding patterns of 18 AFLP primer combinations revealed 264 polymorphic bands. A total of 264 polymorphic fragments were scored with an average of 14.7 fragments per primer combination. The five retro/AFLP primer combinations generated 42 clearly scorable polymorphic bands across 45 genotypes. The number of polymorphic fragments for each primer pair varied from 5 to 12 with an average of 8.4 polymorphic fragments per primer combination. The cluster analysis discriminated the accessions based on the species and the origin of accessions and demonstrated a high level of genetic diversity in?Hyoscyamussp. accessions. According to cluster analysis, almost all accessions ofHyoscyamus niger?and all accessions of?Hyoscyamus reticulatus?were placed in the same group and the accessions of?Hyoscyamus puccillus?from the Yazd province were classified in the same group. These results provide important information with regard to future domestication and breeding programs for management of germplasm resources.
机译：Hyoscyamus？sp。？是两种主要的Tropan生物碱的天然来源，包括Hycycyamine和scopolamine。环境条件使得这种草药在伊朗的种类非常广泛。进行这项研究以评估一组伊朗45个Hyhycyamus？sp的遗传多样性。使用扩增的片段长度多态性和retro / AFLP标记。还使用了18种AFLP标记引物组合和5种Retro / AFLP引物组合来评估种质间的遗传多样性。 18种AFLP引物组合的条带分析显示264条多态性条带。总共对264个多态片段进行评分，每个引物组合平均14.7个片段。五个retro / AFLP引物组合在45个基因型上产生了42条清晰可分的多态性条带。每个引物对的多态性片段的数目在5至12之间变化，每个引物组合平均有8.4个多态性片段。聚类分析根据种的种类和来源对种质进行了区分，并证明了假丝酵母具有较高的遗传多样性。加入。通过聚类分析，将几乎所有的黑霉菌和网状的假丝酵母都归为同一类，而来自亚兹德省的真丝假丝酵母的归类为同一类。这些结果提供了有关未来种质资源管理的驯化和育种计划的重要信息。

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