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In vitro regeneration of Gerbera jamesonii cv. Sunglow

机译:非洲菊的体外再生。松洛

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This?study?demonstrates a protocol for?in vitro?regeneration of?Gerbera jamesonii?cv. ‘Sunglow’ developed by culturing leaf segments on?Murashige and Skoog (MS)medium supplemented with 1.0, 1.5, 2.0, 2.5 and 3.0 mg L-1?indole-3-butyric acid (IBA),?naphthalene acetic acid (NAA),?2,4-dichlorophenoxy acid (2,4-D)?and 1.0, 2.0, 3.0, 4.0 and 5.0 mg L-1?benzyl adenine (BAP)?and?kinetin (Kin). The treatment, 1.5 mg l-1?2,4-D gave 100% callus induction with a friable, nodular and creamish white callus. BAP gave fair callus growth with compact and brownish callus. However, Kin failed to produce callus. Shoot regeneration was assayed with 1.0, 2.0, 3.0, 4.0 and 5.0 mg L-1BAP and Kin. BAP at 3.0 mg l-1?showed 76.67% shoot regeneration, 4 shoots per explant and shoot length of 9 cm, whereas?same concentration of Kin gave only 16.67% regenerated shoots, 1.97 shoots per explant and length of 5.167 cm.?In vitroroot induction was determined by using 0.5, 1.0, 1.5 and 2.0 mg L-1?indole-3-acetic acid (IAA)?and NAA. IAA at 1.5 mg L-1?exhibited a rooting percentage of 97.67%, whereas similar concentration of NAA gave relatively less rooting percentage of 60.67%. IAA gave thick roots with maximum root number (7.567/per explant) and length (7.33 cm), on the contrary, NAA gave relatively thin roots having less number of 3.567 roots with a length of 4.667 cm.
机译:这项研究表明了非洲菊的体外再生协议。通过在补充了1.0、1.5、2.0、2.5和3.0 mg L-1?吲哚-3-丁酸(IBA),萘乙酸(NAA)的Murashige和Skoog(MS)培养基上培养叶段而开发的'Sunglow' ,α,2,4-二氯苯氧基酸(2,4-D)α和1.0、2.0、3.0、4.0和5.0mgL-1α苄基腺嘌呤(BAP)β和肌动蛋白(Kin)。 1.5mgI-1β2,4-D处理产生100%的愈伤组织诱导,具有脆的,结节状和乳白色的愈伤组织。 BAP使紧凑的棕褐色的愈伤组织生长公平。然而,健未能产生愈伤组织。用1.0、2.0、3.0、4.0和5.0mg L-1BAP和Kin测定枝条再生。 BAP浓度为3.0 mg l-1?时显示出76.67%的嫩芽再生,每株外植体4个芽,茎长为9 cm,而Kin的相同浓度仅产生16.67%的再生芽,每株外植体1.97株,长为5.167 cm。通过使用0.5、1.0、1.5和2.0 mg L-1?吲哚-3-乙酸(IAA)?和NAA来测定玻璃根诱导。 IAA 1.5 mg L-1?的生根百分比为97.67%,而相似浓度的NAA的生根百分比则相对较低,为60.67%。 IAA产生的根粗,最大根数为(7.567 /每个外植体),长度为(7.33 cm),相反,NAA产生的根较薄,根数为3.567根,根长为4.667 cm。

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