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首页> 外文期刊>African Journal of Biotechnology >Agar alternatives for micropropagation of African violet (Saintpaulia ionantha)
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Agar alternatives for micropropagation of African violet (Saintpaulia ionantha)

机译：非洲紫罗兰（Saintpaulia ionantha）微繁殖的琼脂替代品

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摘要

Agar is one of the most popular solidifying agents in plant tissue culture. High price of pure grade agar and fear of over exploitation of its resources caused searching for low cost alternatives. In this study, liquid medium with cotton substratum and different combinations of starch, semolina, potato powder and agar in two steps of micropropagation (shoot induction and proliferation) were investigated. The highest frequency of regeneration was found in media containing agar (0.8%), combination of starch: semolina: potato powder (2:1:1) in 9 and 12% and combination of starch (6%) plus agar (0.4%), but maximum shoot numbers were produced in media containing agar (0.8%), combination of starch (6%) plus agar (0.4%) and liquid medium with cotton substratum. The best shoot proliferation take place in liquid medium with cotton substratum. The results show that the combination of starch: semolina: potato powder (2:1:1) in 9% and starch (6%) plus agar (0.4 %) can be suitable alternatives for agar in regeneration stage but the shoot number is lower than agar alone. These options are very cheaper than agar. The best shoot proliferation can be done in bioreactors or liquid medium with suitable substratum like cotton.
机译：琼脂是植物组织培养中最受欢迎的固化剂之一。纯级琼脂的高价格和对资源过度开发的担心导致寻找低成本替代品。在这项研究中，研究了具有棉质基质和淀粉，粗面粉，马铃薯粉和琼脂在微繁育的两个步骤（芽诱导和增殖）中不同组合的液体培养基。在含琼脂（0.8％），淀粉：粗面粉：马铃薯粉（2：1：1）的组合中分别占9％和12％以及淀粉（6％）和琼脂（0.4％）的组合中发现了最高的再生频率，但在含有琼脂（0.8％），淀粉（6％）加琼脂（0.4％）和液体培养基以及棉质基质的培养基中产生的最大芽数。最好的芽增殖发生在带有棉质基质的液体培养基中。结果表明，淀粉（粗粉：马铃薯粉（2：1：1））（9％）和淀粉（6％）加琼脂（0.4％）的组合可能是再生阶段琼脂的合适替代品，但芽数较低而不是琼脂。这些选择比琼脂便宜得多。最好的芽增殖可以在生物反应器或具有合适基质（如棉花）的液体培养基中完成。

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