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Effects of some Iranian Trichoderma isolates on maize seed germination and seedling vigor

机译:伊朗木霉菌某些分离株对玉米种子萌发和幼苗活力的影响

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Trichoderma?species are commonly used as biological control agents against phytopathogenic fungi and some of their isolates are able to improve plant growth. In the current study, we evaluated some Iranian?Trichoderma?isolate cultural filtrates as well as their direct effect on seed germination and seedling vigor of maize (Zea mays?L. cultivar B73); they are?Trichoderma harzianum?T 969,?T. harzianum?T 447,Trichoderma hamatum?T 614,?Trichoderma roseum?T678,?Gliocladium virens?G525 and the unknown?Trichoderma?species isolate (Trichoderma?sp. T) obtained from the soil of Moghan Area, Ardabil Province of Iran. The culture filtrates reduced the speed of seed germination, but no influence (p≥0.05) was recorded at the final rate of the seed germination. When maize seeds were exposed to the?Trichodermaspore suspension, all the?Trichoderma?isolates colonized the seed surface and inhibited the seed germination. The maize seed potted in inoculated soil did not emerge from the soil among all tested?Trichoderma?isolates, except for non-inoculated soil 30 days after potting. When?Trichoderma?conidia were added on the surface of soil near the emerged seed, significant (p≤0.01) decrease of seedling treated separately with?T. hamatum?T614 isolate was observed on the leaves area, fresh root and shoot weight, compared to the non-inoculated seedling. Whereas?T. hamatum?T447 and?T. harzianum?T969 reduced markedly (p≤0.01), seedling fresh root weight and fresh shoot weight were respectively increased as compared to the control.?Trichoderma?isolates had no influence on chlorophyll content in leaves and root length as well as stomata conductivity, except for?Trichoderma?spp.?isolate T that increased stomata conductivity of seedling significantly (p≤0.01).
机译:木霉属种通常用作抗植物病原性真菌的生物防治剂,其某些分离株能够改善植物的生长。在当前的研究中,我们评估了一些伊朗木霉分离培养液,以及它们对玉米种子萌发和幼苗活力的直接影响(Zea mays?L。cultivar B73)。它们是哈茨木霉(Trichoderma harzianum)969,T。 harzianum?T 447,H。Triatumderma hamatum?T 614,rose?Trichoderma?roseum?T678,?Gliocladium v​​irens?G525和未知的Trichoderma?种分离株(Trichoderma?sp。T)是从伊朗Ardabil省Moghan地区的土壤中获得的。培养滤液降低了种子发芽的速度,但没有记录到最终发芽率的影响(p≥0.05)。当玉米种子暴露于木霉孢子悬浮液中时,所有木霉分离物都定居在种子表面并抑制了种子发芽。在所有测试的木霉菌分离物中,接种土壤中的玉米种子均未从土壤中出来,除了在接种后30天未接种土壤外。当在出苗种子附近的土壤表面添加木霉分生孢子时,用ΔT单独处理的幼苗明显减少(p≤0.01)。与未接种苗相比,在叶面积,鲜根和苗重上观察到了Hamatum?T614分离株。鉴于? hamatum?T447和?T。 Harzianum?T969与对照相比显着减少(p≤0.01),幼苗的鲜根重和鲜芽重分别增加。木霉菌分离物对叶片的叶绿素含量,根长和气孔电导率没有影响。用于木霉属分离菌T,可显着提高幼苗的气孔导度(p≤0.01)。

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