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首页> 外文期刊>African Journal of Biotechnology >Impact of polyphenolic extracts on resistance to fungal contamination in dry bean grains
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Impact of polyphenolic extracts on resistance to fungal contamination in dry bean grains

机译：多酚提取物对豆类干粮抗真菌污染的影响

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From their significant roles against the biotic and abiotic stresses, polyphenols have aroused a growing interest for a possible application in food industry. The aim of this study was the evaluation of antifungal activity of polyphenols extracted from two varieties of dry bean, presenting white (Tima) and red (MGT djedida) color. Determination of moisture for the two varieties showed that these grains are favorable for moulds growth. Purification and microscopic study of the isolated strains from beans allowed the identification of six moulds genera:?Alternaria,?Aspergillus,Moniliella,?Fusarium,?Penicillium?and?Rhizopus.?Extraction of total polyphenols was carried by a polar solvent and their quantification was based on the reaction of Folin Ciocalteu. The total polyphenolic content was 0.40 ± 0.005 mg EAG/g for the red variety and 0.27 ± 0.005 mg EAG/g for the white variety.The antifungal activity carried by the methods of direct contact, dilution, and antifungal index (IA100) demonstrated the antifungal capacity of polyphenols; most sensitive strains isolated are?Alternariasp.,?Moniliella?sp. and?Rhizopus?sp..?The most active polyphenols are polyphenols extracted from presumably healthy red grains. Our results lend support of the creation of varieties bean high in polyphenols, which act as natural preservatives and bio-effective agents, and offer an alternative to chemical agents for protection of harvested beans in storage structures.
机译：由于其对生物和非生物胁迫的重要作用，多酚引起了人们对于食品工业中可能应用的兴趣。这项研究的目的是评估从两种干豆中提取的多酚类化合物的抗真菌活性，该多酚类化合物呈现白色（提马）和红色（MGT吉迪达）颜色。测定两个品种的水分表明这些谷物有利于霉菌的生长。对豆中分离出的菌株进行纯化和显微研究后，可以鉴定出六个霉菌属：？Alternaria，？Aspergillus，Moniliella，？Fusarium，？Penicillium？和？Rhizopus。？总多酚的提取是通过极性溶剂进行的，并对其进行了定量是基于Folin Ciocalteu的反应。红色品种的总多酚含量为0.40±0.005 mg EAG / g，白色品种的总多酚含量为0.27±0.005 mg EAG / g。通过直接接触，稀释和抗真菌指数（IA100）进行的抗真菌活性表明多酚的抗真菌能力;分离出的最敏感的菌株是？Alternariasp。，？Moniliella？sp。最活跃的多酚是从大概健康的红色谷物中提取的多酚。我们的结果为创建多酚含量高的豆品种提供了支持，这些品种可作为天然防腐剂和生物有效剂，并为保护存储结构中收获的豆类提供了化学试剂的替代品。

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