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首页> 外文期刊>African Journal of Biotechnology >In vitro induction of tuber formation for the synthesis of secondary metabolites in Chlorophytum borivilianum Sant. et Fernand
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In vitro induction of tuber formation for the synthesis of secondary metabolites in Chlorophytum borivilianum Sant. et Fernand

机译：体外诱导块茎形成，用于合成Sophophytum borivilianum Sant中的次生代谢产物。 et Fernand

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Chlorophytum borivilianum?Sant. et Fernand an endangered herb is valued for several medicinal properties in its tuberous roots. An efficient and reproducible method for inducing?in vitro?tubers from stem disc explant has been developed. Stem disc possessing shoot buds were induced to develop multiple shoots in Murashige and Skoog (MS) medium supplemented with vitamins, 3% sucrose, 0.8% agar and 5?mg/L benzylaminopurine (BAP). Healthy regenerated shoots were rooted in MS basal medium containing 3% sucrose (w/v), 0.8% agar supplemented with?indole-3-acetic acid?(2 mg/L). On further sub culturing, the maximum percentage of tuber formation was obtained in growth hormone free half (?) MS liquid media supplemented with vitamins and 1.5% sucrose after 8 to 9 weeks. The saponin contents of the?in vitroand?in vivo?raised tubers were qualitatively and quantitatively analyzed by?high-performance liquid chromatography?(HPLC) and liquid chromatography?electrospray ionization mass spectrometry?(LC-ESI-MS). There was a significant similarity in the saponins in both tubers. The?in vitro?raised tubers showed similar high metabolite content than?in vivo?grown tubers which is required for medicinal applications. The rooted plantlets were transferred to peat and sand (2:1) with more than 80% success. This is the 1st?report of?in vitro?tuber formation and secondary metabolites screening of?C. borivilianum. This work will give a strong impetus to the pharmaceutical and neutraceutical sectors.
机译：吊兰吊兰？ et al Fernand一种濒临灭绝的草药因其块根具有多种药用价值。已经开发出一种有效且可重复的方法，可以从茎盘外植体中诱导出块茎。在补充了维生素，3％蔗糖，0.8％琼脂和5？mg / L苄氨基嘌呤（BAP）的Murashige和Skoog（MS）培养基中，诱导具有茎芽的茎盘发育成多芽。将健康的再生芽生根于含有3％蔗糖（w / v），0.8％琼脂和2吲哚-3-乙酸（2 mg / L）的MS基础培养基中。在进一步的继代培养中，在8至9周后，在补充了维生素和1.5％蔗糖的无生长激素的半（MS）MS液体培养基中获得了最大的块茎形成百分比。通过“高效液相色谱”（HPLC）和液相色谱“电喷雾电离质谱”（LC-ESI-MS）定性和定量地分析“体外和体内培养的块茎中的皂苷含量”。两种块茎中的皂苷都有显着相似性。体外培养的块茎显示出与体内生长的块茎相似的高代谢产物含量，这是药用的。将生根的小植株转移到泥炭和沙子（2：1），成功率超过80％。这是体外薯块形成和二级代谢产物筛选的第一份报告。牛肝菌。这项工作将极大地推动制药和营养保健领域的发展。

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