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首页> 外文期刊>African Journal of Biotechnology >Comparison of artificial inoculation methods for studying pathogenesis of Alternaria brassicae (Berk.) Sacc on Brassica juncea (L.) Czern. (Indian mustard)
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Comparison of artificial inoculation methods for studying pathogenesis of Alternaria brassicae (Berk.) Sacc on Brassica juncea (L.) Czern. (Indian mustard)

机译：比较用于研究芥菜油菜（B. Czern）上的欧洲链格孢菌（Berk。）Sacc发病机理的人工接种方法的比较。 （印度芥末）

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Establishment of disease by artificial inoculation is essential for studies of various aspects of plant pathology. Keeping this in mind, five inoculation methods were compared namely spraying, infiltration, wounding, spore suspension drop and spore suspension drop along with agarose method. The findings of the present study suggest that out of five inoculation methods used, spore suspension drop along with agarose inoculation method was most ideal as this fixed the inoculum on the target site. The mean value of number of initial disease lesions in drop plus agarose method was highest in all of the time intervals of observation namely 312.2, 484.2, 664.2, 734.2 and 799.2 at 24, 48, 72, 96 and 120 h after pathogen inoculation compared to other methods respectively. Statistical analysis software (SAS) was used to find out the significant comparison among the different methods. The spore suspension drop along with agarose method has the advantage of being accurate and precise, and it was also easy to handle the inoculated plants.
机译：通过人工接种建立疾病对于研究植物病理学的各个方面至关重要。牢记这一点，比较了五种接种方法，即喷洒，渗透，伤口，孢子悬液滴和孢子悬液滴以及琼脂糖方法。本研究的发现表明，在使用的五种接种方法中，孢子悬浮液滴加琼脂糖接种方法是最理想的，因为这可以将接种物固定在目标部位。与病原菌接种相比，滴加琼脂糖法初始病灶数的平均值在所有观察时间间隔最高，分别为312.2、484.2、664.2、734.2和799.2。其他方法分别。使用统计分析软件（SAS）找出不同方法之间的显着比较。孢子悬浮液滴加琼脂糖法具有准确和精确的优点，并且易于处理接种的植物。

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