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首页> 外文期刊>African Journal of Biotechnology >Production of cellulases by Penicillium sp. in a solid-state fermentation of oil palm empty fruit bunch
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Production of cellulases by Penicillium sp. in a solid-state fermentation of oil palm empty fruit bunch

机译：青霉菌产生纤维素酶。在油棕空果堆的固态发酵中

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摘要

To use oil palm empty fruit bunch (EFB) for cellulase production, a novel fungus was isolated from moistened EFB. This fungus was classified as Penicillium sp. by sequence analysis of the internal transcribing space and it was named Penicillium sp. GDX01. The Penicillium sp. strain secreted cellulases under solid-state fermentation of EFB. The fermentation conditions were optimized for maximal enzyme production. Of the different substrates tested, both EFB and rice straw gave the maximum production of filter paper activity (FPase). Five percent yeast extract and 40-50% initial moisture content were found to be optimal for enzyme production. In addition, the pretreatment of EFB with NaOH before fermentation inhibited the cellulase production of Penicillium sp. GDX01. Saccharification of pretreated EFB by cellulases from Penicillium sp. GDX01 resulted in a more than 80% release of glucose during a 72 h incubation, which is a better result than when using Celluclast 1.5L without the addition of β-glucosidase. Our results show that the cellulases produced by Penicillium sp. GDX01 are more efficient at the saccharification of EFB than Celluclast 1.5 L.
机译：为了使用油棕空果束（EFB）来生产纤维素酶，从潮湿的EFB中分离了一种新型真菌。该真菌被分类为青霉菌。通过对内部转录空间的序列分析，将其命名为Penicillium sp.。 GDX01。青霉菌。菌株在EFB固态发酵下分泌纤维素酶。优化发酵条件以产生最大的酶。在测试的不同基材中，EFB和稻草均能最大程度地提高滤纸活性（FPase）。发现百分之五的酵母提取物和40-50％的初始水分含量是酶生产的最佳选择。此外，发酵前用NaOH对EFB进行预处理可抑制青霉菌的纤维素酶产生。 GDX01。青霉属的纤维素酶将预处理的EFB糖化。 GDX01在72小时的培养过程中导致80％以上的葡萄糖释放，这比使用不添加β-葡萄糖苷酶的Celluclast 1.5L时更好。我们的结果表明，青霉菌产生的纤维素酶。 GDX01在糖化EFB方面比Celluclast 1.5 L更有效。

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