• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Adventitious shoots induction and plant regeneration from cotyledons of watermelon (Citrullus lanatus L.)
【24h】

Adventitious shoots induction and plant regeneration from cotyledons of watermelon (Citrullus lanatus L.)

机译：西瓜（Citrullus lanatus L.）子叶的不定芽诱导和植株再生

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A highly efficient regeneration system is a prerequisite step for successful genetic transformation of watermelon cultivars (Citrullus lanatus L.). The objective of this study was to establish efficient in vitro plant regeneration for three watermelon cultivars. To achieve optimal conditions for adventitious shoot induction, the 5-day-old explants (cotyledon base portion, apical portion and hypocotyl) of three cultivars were placed on MB5 media supplemented with different concentrations and combinations of growth regulators (1.0 to 10.0 mg L-1 6-benzyladenine (BA) and 0 to 1.0 mg L-1 indole acetic acid (IAA)); the explants from seedling of different development stages (0 to 10 d) were cultured on MB5 medium containing 2.0 mg L-1 BA and 0.2 mg L-1 IAA for investigating the effect of age on adventitious shoots initiation; besides, 5-day-old seedlings were grown on optimal regeneration medium supplemented with different concentrations of kanamycin for screening the lowest lethal concentration for adventitious shoots. The results show that the basal region of cotyledon showed higher frequency of shoot formation (79.17-83.33%) than the apical region (5.23-8.25%); high percentage of shoots regeneration was induced from 5-day-old cotyledons base portion cultured on MB5 containing 1 or 2 mg L-1 BA; the 100 mg L-1 kanamycin proved to be the optimal concentration for screening the transformants. Our results provide an efficient stable regeneration system for genetic transformation of watermelon.
机译：高效的再生系统是成功进行西瓜品种（Citrullus lanatus L.）遗传转化的必要步骤。这项研究的目的是为三个西瓜品种建立有效的体外植物再生。为了获得诱导不定芽的最佳条件，将三个品种的5天大的外植体（子叶基部，顶端部分和下胚轴）放在MB5培养基中，添加不同浓度和生长调节剂（1.0至10.0 mg L- 1 6-苄基腺嘌呤（BA）和0至1.0 mg L-1吲哚乙酸（IAA））;将不同发育阶段（0-10 d）幼苗的外植体培养在含有2.0 mg L-1 BA和0.2 mg L-1 IAA的MB5培养基上，以研究年龄对不定芽萌发的影响;此外，在补充有不同浓度卡那霉素的最佳再生培养基上生长5天大的幼苗，以筛选不定芽的最低致死浓度。结果表明，子叶的基部区域的芽形成频率（79.17-83.33％）比顶部区域（5.23-8.25％）高;从在含有1或2 mg L-1 BA的MB5上培养的5天大的子叶基础部分诱导高百分比的芽再生。 100 mg L-1卡那霉素被证明是筛选转化株的最佳浓度。我们的结果为西瓜的遗传转化提供了有效的稳定再生系统。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号