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首页> 外文期刊>African Journal of Biotechnology >Physiological responses by Billbergia zebrina (Bromeliaceae) when grown under controlled microenvironmental conditions
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Physiological responses by Billbergia zebrina (Bromeliaceae) when grown under controlled microenvironmental conditions

机译：在控制的微环境条件下生长的Billbergia zebrina（Bromeliaceae）的生理响应

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摘要

Sucrose, the most commonly used carbon source in conventional in vitro culture, and limited air exchange in the culture containers are factors that affect the growth of in vitro-cultured plants. They may induce physiological disorders and decrease the survival rate of plants after transfer to ex vitro conditions. The aim of the present study was to analyze the effects of gas exchange and sucrose concentration on Billbergia zebrina plantlets during in vitro propagation. In vitro-established B. zebrina plantlets were transferred to culture media containing 0, 15, 30, 45, or 60 g L-1 sucrose. Two different culture-container sealing systems were compared: lids with a filter (permitting gas exchange) and lids with no filter (blocking fluent gas exchange). Carbohydrate and chlorophyll (Chl a+b) concentrations were analyzed in plantlets at 45-days of culture. The addition of sucrose to the medium reduced the Chl a+b concentration in the plantlets. On the other hand, additional sucrose had a positive effect on the carbohydrate stock formation of the plantlets. The results showed that a photoautotrophic system (air exchange and a sugar-free medium) improves the in vitro propagation of B. zebrina without creating physiological disorders.
机译：蔗糖是常规体外培养中最常用的碳源，并且培养容器中的空气交换有限，是影响体外培养植物生长的因素。在转移到离体条件后，它们可能诱发生理疾病并降低植物的存活率。本研究的目的是分析在体外繁殖期间气体交换和蔗糖浓度对Billbergia zebrina苗的影响。将体外建立的斑节芽孢杆菌幼苗转移至含有0、15、30、45或60g L-1蔗糖的培养基中。比较了两种不同的培养容器密封系统：带过滤器的盖子（允许交换气体）和不带过滤器的盖子（阻止流态气体交换）。培养45天时分析幼苗中的碳水化合物和叶绿素（Chl a + b）浓度。向培养基中添加蔗糖降低了幼苗中Chl a + b的浓度。另一方面，额外的蔗糖对幼苗的碳水化合物储备形成有积极作用。结果表明，光合自养系统（空气交换和无糖培养基）可改善斑节杆菌的体外繁殖，而不会造成生理障碍。

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