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首页> 外文期刊>African Journal of Biotechnology >Microsatellites markers associated with resistance to flower bud thrips in a cowpea F2 population derived from genotypes TVU-123 and WC36
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Microsatellites markers associated with resistance to flower bud thrips in a cowpea F2 population derived from genotypes TVU-123 and WC36

机译：基因型TVU-123和WC36产生的cow豆F2群体中与花蕾蓟马的抗性相关的微卫星标记

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摘要

Breeding for resistance to flower bud thrips (Megalurothrips sjostedti) in cowpea has been hindered by the quantitative nature of resistance. To identify simple sequence repeat (SSR) markers associated with resistance to flower bud thrips that could be used for marker-assisted breeding, a F2 population was generated from a cross between genotypes TVU-123 (resistant) and WC36 (susceptible). The population was evaluated for thrips damage scores, thrips counts, and pods number per plant under artificial infestation. Sixty-six microsatellites markers were screened between the two parental lines and seven polymorphic markers were used to genotype 100 F2 plants. Single marker analysis was used to evaluate an association between the markers and traits. Transgressive segregation among the F2 plants for resistance to flower thrips was observed. A significant negative relationship was observed between thrips damage scores and pods number per plant. Markers CP37/38 and CP215/216 were significantly associated with thrips damage scores and thrips counts, respectively. The two markers explained 7 and 11.2% of the total variation in thrips damage scores and thrips counts with positive and negative effects, respectively. Mainly additive gene effects were observed. A more detailed study using more markers on these loci should provide better understanding of this complex trait.
机译：抗性的定量特性阻碍了cow豆对花蕾蓟马（Megalurothrips sjostedti）的抗性育种。为了鉴定与可用于标记辅助育种的对花蕾蓟马的抗性相关的简单序列重复（SSR）标记，从TVU-123（抗性）和WC36（易感）基因型之间的杂交中产生了F2群体。对该种群进行了人工侵染后的蓟马危害评分，蓟马数量和荚果数量的评估。在两个亲本系之间筛选了66个微卫星标记，并使用7个多态性标记对100 F2植物进行基因分型。使用单标记分析来评估标记与性状之间的关联。观察到F2植物之间的过分隔离对花蓟马的抗性。在每株植物的蓟马损伤评分和豆荚数量之间观察到显着的负相关。标记CP37 / 38和CP215 / 216分别与蓟马损伤评分和蓟马计数显着相关。这两个标记分别解释了蓟马损伤得分和蓟马计数总体变化的7％和11.2％，分别具有正面和负面影响。主要观察到加性基因效应。在这些基因座上使用更多标记的更详细的研究应提供对这种复杂性状的更好理解。

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