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首页> 外文期刊>African Journal of Biotechnology >Progress in strategies for sequence diversity library creation for directed evolution
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Progress in strategies for sequence diversity library creation for directed evolution

机译：用于定向进化的序列多样性文库创建策略的进展

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Protein engineering has been the most attractive strategy for biologists to redesign enzymes. As the simplest technique of protein engineering, directed evolution has been applied to many fields, such as industry, agriculture and medicine. An experiment of directed evolution comprises mutant libraries creation and screening or selection for enzyme variants with desired properties. Therefore, a successful application of directed evolution depends on whether or not one can generate a quality library and perform effective screening to find the desired properties. Directed evolution is already increasingly used in many laboratories to improve protein stability and activity, alter enzyme substrate specificity, or design new activities. Meanwhile, many more effective novel strategies of mutant library generation and screening or selection have emerged in recent years, and will continue to be developed. Combining computational/rational design with directed evolution has been developed as more available means to redesign enzymes.
机译：蛋白质工程一直是生物学家重新设计酶的最有吸引力的策略。作为蛋白质工程最简单的技术，定向进化已应用于许多领域，例如工业，农业和医学。定向进化的实验包括创建突变体文库以及筛选或选择具有所需特性的酶变体。因此，定向进化的成功应用取决于能否生成质量库并进行有效筛选以找到所需的特性。定向进化已在许多实验室中越来越多地用于改善蛋白质的稳定性和活性，改变酶底物的特异性或设计新的活性。同时，近年来出现了许多更有效的突变体文库产生，筛选或选择的新颖策略，并且将继续发展。已经将计算/合理设计与定向进化相结合，作为重新设计酶的更多可用手段。

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