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首页> 外文期刊>African Journal of Biotechnology >In vitro antigenotoxic and anti-oxidative capacity of Hypnea musciformis (Wulfen) Lamouroux extract in human lymphocytes
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In vitro antigenotoxic and anti-oxidative capacity of Hypnea musciformis (Wulfen) Lamouroux extract in human lymphocytes

机译：麝香呼吸道提取物对人淋巴细胞的体外抗原毒性和抗氧化能力

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摘要

Several hazardous substances, can damage our DNA in various ways.?Defining these substances and the protective ones is very important.?Therefore, researches have increased on various compounds based on natural sources which keep the harmful effects of these various agents at the minimum level.?In the present study, we evaluated the potential genotoxic/ antigenotoxic/ antimutagenic activity of the crude chloroform: methanol (2:1) extracts of?Hypnea musciformis?(Wulfen) Lamouroux (HME), in human lymphocytes culture?in vitro?against genotoxic/mutagenic agents mitomycin C (MMC) and ethyl methanesulfonate (EMS) by using chromosome aberration (CA), sister chromatid exchange (SCE) and micronuclei (MN) assays, and also determined total antioxidant capacity (in soluble lipid and water), phenolic?compound,?protein, carbohydrate, vitamins (A, C and E) contents. The frequency of chromosome aberrations and SCE increased by MMC, were significantly decreased by HME (p < 0.05 for CA, p?< 0.001 for SCE). The MN frequencies of the cells were significantly decreased by the treatment with HME plus MMC or EMS when compared with the positive controls (MMC or EMS) (p < 0.05).?In conclusion, HME itself is not a clastogenic or cytotoxic substance. On the other hand, HME possesses a strong antigenotoxic, anti-clastogenic and protective effects against MMC?in vitro.
机译：几种有害物质会以各种方式破坏我们的DNA。？定义这些物质和保护性物质非常重要。因此，基于天然来源的各种化合物的研究不断增加，这些化合物将这些物质的有害作用保持在最低水平在本研究中，我们评估了在人淋巴细胞体外培养的氯仿：“ Mypnea musciformis”（Wulfen）Lamouroux（HME）的甲醇（2：1）提取物的潜在遗传毒性/抗原毒性/抗诱变活性。通过使用染色体畸变（CA），姐妹染色单体交换（SCE）和微核（MN）分析来对抗遗传毒性/诱变剂丝裂霉素C（MMC）和甲磺酸乙酯（EMS），还测定了总抗氧化能力（在可溶性脂质和水中） ，酚类化合物，蛋白质，碳水化合物，维生素（A，C和E）含量。 MMC增加了染色体畸变和SCE的频率，而HME则显着降低了频率（CA的p <0.05，SCE的p <0.001）。与阳性对照（MMC或EMS）相比，HME加MMC或EMS处理可显着降低细胞的MN频率（p <0.05）。总而言之，HME本身不是致癌物质或细胞毒性物质。另一方面，HME在体外对MMC？具有很强的抗原毒性，抗破伤性和保护作用。

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