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首页> 外文期刊>African Journal of Biotechnology >Effects of AdR-siPTEN on learning capability, memory and extracellular signal-regulated kinase expression in hippocampus of rats with vascular dementia
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Effects of AdR-siPTEN on learning capability, memory and extracellular signal-regulated kinase expression in hippocampus of rats with vascular dementia

机译：AdR-siPTEN对血管性痴呆大鼠学习能力，记忆力和海马细胞外信号调节激酶表达的影响

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This study investigated the effects of a recombinant adenovirus, AdR-siPTEN, on learning capability, memory and extracellular signal-regulated kinase (ERK) expression in the hippocampus of rats with vascular dementia (VD). VD was introduced via permanent bilateral common carotid artery ligation (2-VO) in rats. AdR-siPTEN recombinant adenovirus and unrelated control adenovirus AdRFP were independently injected into the hippocampus of VD rats. Four weeks later, Morris water maze test was performed to detect the cognition of rats. Hematoxylin and eosin (HE) and Nissle staining were used to observe cellular morphous and neuronal damage in the hippocampus CA1 region. The mRNA and protein expression of a phosphatase and tensin homolog deleted on chromosome 10 (PTEN), phosphatidylinositol 3-kinase (PI3K), ERK and cAMP response element-binding (CREB) were detected by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry, respectively. PTEN expression was reduced apparently in hippocampus after RNAi intervation in the AdR-siPTEN group (P<0.05). Compared with the AdRFP group, rats in the AdR-siPTEN group had significantly shorter escape latency to the invisible platform (except on the first day of test) (P<0.05); and more neurons with regular shape and/or Nissle bodies in hippocampus were observed in the AdR-siPTEN group, suggesting attenuated neuronal damage and degeneration. Additionally, the expression of PTEN , PI3K, ERK and CREB in hippocampus CA1 region of AdR-siPTEN treatment group was markedly higher than that in the AdRFP group (P < 0.05). However, compared with the normal group, the expression of PTEN, PI3K, ERK and CREB was dramatically decreased in the AdRFP group (P < 0.05). AdR-siPTEN may improve the cognition of VD rats, attenuate neuronal damage and promote expression of ERK and CREB, leading to the protective effects on neuronal synaptic plasticity in VD rats. These results suggest that PTEN down-regulation may exert potential therapeutic effects on VD.
机译：这项研究调查了重组腺病毒AdR-siPTEN对血管性痴呆（VD）大鼠海马的学习能力，记忆力和细胞外信号调节激酶（ERK）表达的影响。 VD是通过大鼠永久性双侧颈总动脉结扎术（2-VO）引入的。将AdR-siPTEN重组腺病毒和无关的对照腺病毒AdRFP分别注射到VD大鼠的海马中。四周后，进行莫里斯水迷宫测试以检测大鼠的认知。使用苏木精和曙红（HE）和Nissle染色观察海马CA1区的细胞形态和神经元损伤。通过逆转录聚合酶链反应（RT-PCR）检测10号染色体（PTEN），磷脂酰肌醇3激酶（PI3K），ERK和cAMP反应元件结合（CREB）缺失的磷酸酶和张力蛋白同源物的mRNA和蛋白质表达。和免疫组织化学分别。 RNA干扰后，AdR-siPTEN组海马PTEN表达明显降低（P <0.05）。与AdRFP组相比，AdR-siPTEN组的大鼠向隐身平台的逃避潜伏期显着缩短（测试第一天除外）（P <0.05）;在AdR-siPTEN组中观察到更多的海马具有规则形状和/或Nissle体的神经元，提示神经元损伤和变性减弱。另外，AdR-siPTEN治疗组海马CA1区PTEN，PI3K，ERK和CREB的表达明显高于AdRFP组（P <0.05）。然而，与正常组相比，AdRFP组的PTEN，PI3K，ERK和CREB的表达明显降低（P <0.05）。 AdR-siPTEN可能改善VD大鼠的认知，减轻神经元损伤并促进ERK和CREB的表达，从而导致对VD大鼠神经元突触可塑性的保护作用。这些结果表明，PTEN的下调可能对VD发挥潜在的治疗作用。

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