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首页> 外文期刊>African Journal of Biotechnology >Characterization of rhodanese produced by Pseudomonas aeruginosa and Bacillus brevis isolated
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Characterization of rhodanese produced by Pseudomonas aeruginosa and Bacillus brevis isolated

机译：铜绿假单胞菌和短芽孢杆菌分离出的若丹丹的特征

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Wastewater from numerous cassava processing industries contains cyanide which is toxic to several forms of life. This effluent constitutes serious hazards to the environment and aquatic life in receiving water bodies. Enzymatic remediation of polluted environment presents advantages over traditional technologies and also over microbial remediation. Extracellular rhodanese of strains of?Pseudomonas aerugionosa?and?Bacillus brevis?isolated from soil of cassava processing site were studied. Biochemical characteristics of the purified enzymes, including pH and temperature profiles were also determined. Production of rhodanese correlated with the rate of bacterial growth at the exponential phase for both strains. Optimum incubation time for maximum enzyme production was 20 h in both cases. The enzyme was purified 9.72-fold with a yield of 37.8% and specific activity of 6.32 mg-1protein in the case of?P. aeruginosa. The enzyme from?B. brevis?was purified 7.44-fold with a yield of 22.6% and specific activity of 5.21 mg-1?protein.? The enzyme generally demonstrated a broad pH range but optimum pH was 6.0 and 7.0 for?P. aeruginosaand?B. brevis?rhodanese activity respectively. Optimum temperatures for?P. aeruginosa?and?B. brevis?rhodanese were 50 and 40°C, respectively, with both enzymes retaining activity up to 70°C. The Km values for KCN and Na2S2O3?as substrates for?P. aeruginosa?rhodanese were 12.5 and 0.0066 mM, respectively, while the Km values for the same substrates for?B. brevis?rhodanese were 3.12 and 11.1 mM, respectively. Cations, Hg2+, Ba2+, and Co2+?all inhibited the enzyme activities of both bacteria. The bacteria rhodaneses have potential for effective remediation of cyanide-polluted environments, ultimately leading to improvement of fish and other aquatic organisms in receiving water bodies.
机译：来自许多木薯加工业的废水中含有氰化物，对多种生命形式都有毒。该废水对接收水体构成环境和水生生物的严重危害。对污染环境的酶法修复优于传统技术，也优于微生物修复。研究了从木薯加工区土壤中分离到的铜绿假单胞菌和短芽孢杆菌的胞外罗丹花。还确定了纯化酶的生化特性，包括pH和温度曲线。对于两种菌株，若丹红花的产生与细菌在指数期的生长速率相关。在两种情况下，最大酶产生的最佳孵育时间均为20小时。在ΔP情况下，该酶被纯化了9.72倍，产率为37.8％，比活性为6.32mg-1蛋白。铜绿。 B酶。 brevis？被纯化了7.44倍，产率为22.6％，比活性为5.21 mg-1？蛋白。该酶通常显示出较宽的pH范围，但对于ΔP而言，最佳pH为6.0和7.0。铜绿假单胞菌？ brevis？rhodanese活动分别。 P的最佳温度。铜绿和？ brevis？rhodanese分别为50和40°C，两种酶的活性最高可保持70°C。 KCN和Na2S2O3？的Km值作为？P的底物。铜绿罗丹色铜分别为12.5和0.0066 mM，而相同底物的Bm的Km值。 brevis？rhodanese分别为3.12和11.1 mM。阳离子，Hg2 +，Ba2 +和Co2 +？均抑制两种细菌的​​酶活性。罗丹素细菌具有有效修复氰化物污染环境的潜力，最终导致鱼类和其他水生生物在接收水体中得到改善。

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