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首页> 外文期刊>African Journal of Biotechnology >Seed germination and in vitro plant regeneration of Parkia biglobosa (Jacq.) Benth
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Seed germination and in vitro plant regeneration of Parkia biglobosa (Jacq.) Benth

机译：大叶柏（Parkia biglobosa，Jacq。Benth）的种子萌发和离体植株再生

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Parkia biglobosa?is an important leguminous forest species which is being threatened of going into extinction in Senegal. To preserve this genetic resource of great economic value, studies on germination were carried out and?in vitroconservation option through tissue culture technique was adopted. 100% of germination rate was recorded 2 days after 5 - 6 h of concentrated H2SO4treatments and incubation in a growth chamber at 27±1°C. To improve and raise?in vitro?vegetative multiplication capacities, different concentrations of growth regulators were added alone or in combination in a MS basal medium. Multiplication rate of 3.08 and 3.29 were obtained, respectively, for apex and cotyledonary nodes in a MS medium supplemented with BAP 0.5 mg.L-1?+ TIBA 0.1 mg.L-1. For axillary explants, a rate of 3.58 was recorded for the hormonal combination which consisted of BAP 0.5 mg.L-1?+ TIBA 0.5 mg.L-1?+ AgNO3?0.5 mg.L-1. 41.66% of apex and 58.33% of cotyledonary explants were rooted after 24 h of induction treatment with IBA 2.5mg.L-1?whereas 41.66% of axillary bud explants developed roots after 48 h of induction with NAA 1mg.L-1. During acclimatization achievement, survival rates were respectively 80% for apex and cotyledonary explants and 86.66% for axillary explants.
机译：大头柏（Parkia biglobosa）是一种重要的豆科林种，正受到威胁在塞内加尔灭绝。为了保护这一具有重大经济价值的遗传资源，进行了萌发研究，并采用组织培养技术进行了体外保存。浓缩H2SO4处理5-6小时并在27±1°C的生长室中孵育2天后，记录100％的发芽率。为了提高和提高体外营养繁殖能力，可以在MS基础培养基中单独或组合添加不同浓度的生长调节剂。在补充了BAP 0.5 mg.L-1？+ TIBA 0.1 mg.L-1的MS培养基中，顶点和子叶节的增殖率分别为3.08和3.29。对于腋窝外植体，激素组合的记录速率为3.58，该组合由BAP 0.5 mg.L-1？+ TIBA 0.5 mg.L-1？+ AgNO3？0.5 mg.L-1组成。用IBA 2.5mg.L-1诱导处理24小时后，有41.66％的先端和58.33％的子叶外植体生根，而用NAA 1mg.L-1诱导48h后，有41.66％的腋芽外植体形成了根。在适应过程中，根尖和子叶外植体的成活率分别为80％和腋窝外植体的成活率分别为86.66％。

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