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首页> 外文期刊>African Journal of Biotechnology >Suitability and use of two molecular markers to track race-specific resistance striga gesnerioides in cowpea (Vigna unguiculata (L.) Walp.)
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Suitability and use of two molecular markers to track race-specific resistance striga gesnerioides in cowpea (Vigna unguiculata (L.) Walp.)

机译：在cow豆中（Vigna unguiculata（L.）Walp。）适合和使用两种分子标记来追踪种族特异性抗性gerges gernerioides。

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The obligate root parasitic weed Striga gesnerioides poses a severe constraint to cowpea productivity in the dry savannahs of West and Central Africa, where cowpea is a major crop. At least seven races of S. gesnerioides have been identified within the cowpea-growing regions of West and Central Africa, based on host differential response and genetic diversity analysis. Molecular markers linked to resistance to different races of S. gesneriodes have been identified. It was desirable to demonstrate the applicability and efficiency of such markers for use in marker-assisted selection (MAS) to fast-track the development of cowpea with resistance to S. gesnerioides. The objective of the study was to determine the suitability of two molecular markers in tracking resistance in cowpea to S. gesnerioides race SG3, the predominant race found in Nigeria. F2 mapping populations and recombinant inbred lines (RILs) derived from the cross involving IT97K-499-35 and a susceptible local landrace (Borno Brown), and another resistant parent B301 with the same susceptible land race (Borno Brown) were assayed using two linked markers. Genetic analysis showed that resistance to S. gesnerioides in cowpea is inherited as a single dominant gene. Two SCAR markers, 61RM2 and C42-2B were validated in the same F2 populations and subsequent recombinant inbred lines (RILs). The two markers were able to discriminate between resistance and susceptibility and the genotypic score was quite similar to the phenotypic score with the markers score showing greater efficiency in selection than phenotypic score. The 61RM2 had two bands in resistant cultivars and amplified a ~450 bp fragment with marker efficiency of 98% while C42-2B amplified a single ~250 bp fragment with marker efficiency of 96% in resistant cultivars and absent in susceptible cultivars. The genetic distance between 61RM2 and phenotypic score was 3.5 cM while that of C42-2B and phenotypic score was 8.5 cM. The two marker data set were significantly correlated with the phenotypic data (r=0.95). Based on the tight linkage with the resistant locus, 61RM2 was found to be a utility marker to initiate MAS in cowpea breeding for resistance to S. gesnerioides.
机译：西非和中非干旱大草原上，专性根寄生杂草Striga gesnerioides严重限制了productivity豆的生产力，而cow豆是主要农作物。根据寄主的差异反应和遗传多样性分析，在西非和中非的pea豆生长区域内，至少鉴定出7个种族的gers。gersnerioides。已经鉴定了与对格氏链球菌不同种族的抗性相关的分子标记。期望证明这些标记物在标记物辅助选择（MAS）中的应用性和效率，以快速追踪对格氏链球菌具有抗性的cow豆的发育。该研究的目的是确定两种分子标记物在追踪cow豆对格氏链球菌SG3（在尼日利亚发现的主要种族）的抗性中的适用性。使用两个关联分析法测定了F2作图种群和来源于涉及IT97K-499-35和易感本地长白（Borno Brown）的杂交的重组自交系（RIL），以及具有相同易感陆地种族的另一个抗性亲本B301（Borno Brown）。标记。遗传分析表明，cow豆中对格氏链球菌的抗性是作为单个显性基因遗传的。在相同的F2群体和随后的重组自交系（RIL）中验证了两个SCAR标记61RM2和C42-2B。这两个标记能够区分耐药性和易感性，并且基因型评分与表型评分非常相似，其中标记评分显示的选择效率高于表型评分。 61RM2在抗性品种中有两个条带，扩增了一个〜450 bp的片段，标记效率为98％，而C42-2B在抗性品种中扩增了一个〜250 bp的片段，标记效率为96％，而在易感品种中不存在。 61RM2与表型评分之间的遗传距离为3.5 cM，而C42-2B与表型评分之间的遗传距离为8.5 cM。这两个标记数据集与表型数据显着相关（r = 0.95）。基于与抗性基因座的紧密联系，发现61RM2是在cow豆育种中针对鼠疫链球菌抗性启动MAS的有用标记。

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