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首页> 外文期刊>African Journal of Biotechnology >Enrichment and isolation of microbial strains degrading bioplastic polyvinyl alcohol and time course study of their degradation potential
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Enrichment and isolation of microbial strains degrading bioplastic polyvinyl alcohol and time course study of their degradation potential

机译：降解生物塑料聚乙烯醇的微生物菌株的富集和分离及其降解潜力的时程研究

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Polyvinyl alcohol (PVA) degrading bacterial strains were isolated from various environmental sites rich in plastic wastes by using the enrichment culture technique. Among the various isolated strains, the selected potent PVA degrading bacterial strains were tentatively characterized as Bacillus and Pseudomonas sp. The time course of the PVA degradation potential of the characterized strains in growth media containing PVA as a major carbon source was evaluated using the spectropho-tometric assay method. This was done by determining the residual PVA remaining in the culture media, increase in cell growth and change in pH of the media over a period of twenty days. The ultimate biodegradation (mineralization) of PVA to its mineral constituents CO2 and H2O was determined by the CO2 evolution test. The strain characterized as Bacillus sp. showed 65% of PVA degradation as determined by spectrophotometric assay and 45.4% of mineralization of PVA over a period of 20 days. The strain characterized as Pseudomonas sp. showed 42% of PVA degradation as determined by spectrophoto-metric assay and 28.9% of mineralization of PVA over a period of 20 days.
机译：通过使用富集培养技术，从富含塑料废料的各种环境场所中分离出降解聚乙烯醇（PVA）的细菌菌株。在各种分离的菌株中，选定的有效降解PVA的细菌菌株暂定为芽孢杆菌和假单胞菌。使用分光光度法测定了特征菌株在含有PVA作为主要碳源的生长培养基中的PVA降解潜能的时程。通过确定在20天的时间内培养基中残留的PVA，细胞生长的增加和pH的变化来完成。通过CO2析出试验确定PVA最终生物降解（矿化）为其矿物成分CO2和H2O。该菌株的特征为芽孢杆菌。通过分光光度法测定，在20天内PVA降解率达65％，PVA矿化率达45.4％。表征为假单胞菌sp。的菌株。通过分光光度法测定显示PVA降解42％，在20天内PVA矿化28.9％。

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