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首页> 外文期刊>African Journal of Biotechnology >Evaluation of genetic diversity in barley (Hordeum vulgare L.) from Wollo high land areas using agro-morphological traits and hordein
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Evaluation of genetic diversity in barley (Hordeum vulgare L.) from Wollo high land areas using agro-morphological traits and hordein

机译：利用农业形态特征和大麦醇溶性激素评估沃洛高地大麦（Hordeum vulgare L.）的遗传多样性

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This study aimed to determine the genetic diversity and relationships among barley varieties (Hordeum vulgare L.) growing at Wollo Highland areas by using hordein and agro-morphological traits. Twenty (20) varieties were laid down in randomized complete block design (RCBD) design with three replications; they were planted by irrigation at Wollo University, Dessie Campus from January to May 2014. The genetic analysis using hordein was done in the laboratory of Ethiopian Biodiversity Institute (EBI) in July 2014. Ten (10) competitive random plants from the rows of the experimental plots were taken for recording their agro-morphological characters. Electrophoretic separation of barley storage proteins or hordeins was done using acid polyacrylamide gel electrophoresis (A-PAGE). The traits: day of heading, day of maturity, grain yield (kg/ha), plant height, spike length, number of spiklet per spike, kernel number per spike, weight of seed per spike and biomass yield (g/plot) were highly significant for the diversity of barely, whereas thousand seed weight was less significant. The results reveal positive correlation between spike length and number of spiklet per spike (the highest correlations from the agro morphological traits); the next highly correlated traits were kernel weight per spike and thousand seed weight. The A-PAGE analysis showed limited variation among the analysed accessions. The Nei’s genetic distance for all varieties of barely varied from 0.0000 to 1.6094. It is found that the 20 genotypes of barely investigated in this research were having a gene diversity (h) of overall populations (0.138) using hordein. The cluster analysis grouped the 20 barely genotypes into three different clusters using agro-morphological traits and into four clusters using hordein. This indicates the presence of wide diversity among the tested genotypes. From cluster mean values of agro-morphological traits, genotypes in cluster III deserve consideration for directly developing high yielding barely varieties. The result of the principal components analysis revealed that the first three principal components having greater than 1 eigenvalue contributed 84.22% of the total variation. From this study, it can be concluded that the presence of high morphological variation indicated the potential of Wollo Highland areas in contributing to barley improvement and conservation activities of land areas.
机译：这项研究旨在通过利用大麦醇溶蛋白和农业形态学特征来确定在沃洛高地地区生长的大麦品种（大麦）的遗传多样性及其相互关系。二十三个（20）品种在随机完整区组设计（RCBD）设计中进行了三个重复。它们于2014年1月至5月在Wollo大学Dessie校区灌溉种植。2014年7月，在埃塞俄比亚生物多样性研究所（EBI）的实验室中使用大麦醇溶蛋白进行了遗传分析。取实验田以记录其农业形态特征。大麦贮藏蛋白或大麦醇溶蛋白的电泳分离采用酸性聚丙烯酰胺凝胶电泳（A-PAGE）进行。性状：抽穗日，成熟日，谷物产量（kg / ha），株高，穗长，每个穗的小穗数，每个穗的粒数，每个穗的种子重量和生物量产量（g /图）为几乎没有多样性，而千粒重不那么重要。结果表明，穗长与每个穗的小穗数之间呈正相关（从农业形态性状来看，相关性最高）。接下来的高度相关性状是每个穗粒重和种子重量千粒。 A-PAGE分析显示分析的种质之间的变化有限。 Nei的所有品种的遗传距离从0.0000到1.6094不等。结果发现，使用大麦醇溶蛋白进行的这项研究中几乎未进行研究的20个基因型在总人群（0.138）中具有基因多样性（h）。聚类分析利用农业形态学特征将20个勉强的基因型分为三个不同的聚类，并利用大麦醇溶蛋白将其分为四个聚类。这表明测试的基因型之间存在广泛的多样性。从农业形态性状的聚类平均值来看，聚类III中的基因型值得直接开发高产勉强品种。主成分分析结果表明，前三个主成分的特征值均大于1，占总变异的84.22％。从这项研究可以得出结论，高形态变化的存在表明Wollo高地地区在促进大麦改良和土地保护活动方面的潜力。

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