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首页> 外文期刊>African Journal of Biotechnology >Production of invertase from Saccharomyces cerevisiae Angel using date syrup as a cost effective carbon source
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Production of invertase from Saccharomyces cerevisiae Angel using date syrup as a cost effective carbon source

机译：使用枣糖浆作为经济有效的碳源，从酿酒酵母天使生产蔗糖酶

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摘要

Invertase (β-fructofuranosidase) finds major applications in food, cosmetics, and pharmaceutical industries. In the present study, fermentation conditions for invertase production from Saccharomyces cerevisiae Angel using date syrup in shake flask were investigated. The effect of time period (0-96 h), carbon sources (sucrose, date syrup, maltose, galactose, and glucose), nitrogen sources including yeast extract, peptone, urea, meat extract, and ammonium sulphate, pH (4 to 6.5), temperature (30 to 40°C), date syrup concentration (5 to 50 g/L), and inoculum size (1 to 10% v/v) were optimized. Optimum conditions for yeast growth and invertase yield were 20 g/L date syrup, 10 g/L yeast extract, temperature of 37°C, pH 5.5, and 60 h fermentation time. In addition, effect of date syrup concentration and size of inoculum were also optimized. Highest invertase titer of ~190 U/mL were obtained when 40 g/L of date syrup and 10% v/v inoculum were used. These results show great practical potential for invertase production by saving enzyme production cost.
机译：转化酶（β-果糖呋喃糖苷酶）在食品，化妆品和制药行业中发现了主要应用。在本研究中，研究了使用摇瓶中的枣糖浆从啤酒酵母中生产转化酶的发酵条件。时间段（0-96小时），碳源（蔗糖，大枣糖浆，麦芽糖，半乳糖和葡萄糖），氮源（包括酵母提取物，蛋白ept，尿素，肉提取物和硫酸铵）的pH值（4至6.5）的影响），温度（30至40°C），大枣糖浆浓度（5至50 g / L）和接种量（1至10％v / v）进行了优化。酵母生长和转化酶产量的最佳条件是20 g / L枣糖浆，10 g / L酵母提取物，37°C温度，pH 5.5和60 h发酵时间。此外，还优化了枣糖浆浓度和接种量的影响。当使用40 g / L的大枣糖浆和10％v / v接种物时，可获得最高〜190 U / mL的转化酶滴度。这些结果表明，通过节省酶的生产成本，具有很大的生产转化酶的实际潜力。

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