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首页> 外文期刊>African Journal of Biotechnology >Isolation of chromium resistant bacteria from a former bauxite mine area and their capacity for Cr (VI) reduction
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Isolation of chromium resistant bacteria from a former bauxite mine area and their capacity for Cr (VI) reduction

机译：从原铝土矿区中分离出耐铬细菌及其还原Cr（VI）的能力

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The Cr (VI) reducing capacity of bacteria has been investigated in many different soils and waters but little or no information is available from soils originating from bauxite mine areas. From soil, mud and rhizospheres of the floating aquatic plantPotamogeton natans?L. and the terrestrial plant?Carduus acanthoides?L., the Cr content was determined and the microbial populations were sampled. The highest total chromium concentration (204.6 mgkg-1) was found in the rhizosphere of?C. acanthoides. To determine the numbers and percentages of chromate-resistant bacteria, the autochthonous microbial populations were subjected to different Cr (VI) concentrations (40, 100, 300 and 1000 mM as K2Cr2O7). At 1000 mM Cr (VI) in the medium, about 25% of bacteria from soil and 45% of bacteria from the rhizospheres were resistant. Of 34 bacterial isolates, within 24 h, only?Bacillusstearothermophilus?12 ms,?Pseudomonas?sp.?12 bk3 and?Serratia fonticola?7 be were able to reduce 50 μM Cr (VI). Using prolonged 72 h incubation, they were able to reduce 500 μM Cr (VI) concentrations added to the medium. These chromate-resistant bacteria isolated from rhizosphere of plants growing in bauxite mine soil have great potential for bioremediation of Cr (VI)-polluted wastes.
机译：已经在许多不同的土壤和水中研究了细菌降低Cr（VI）的能力，但从铝土矿区获得的土壤中几乎没有或没有信息。来自漂浮水生植物的土壤，泥浆和根际并测定陆地植物刺五加（Carduus acanthoides）L.的铬含量，并对微生物种群进行取样。在？C的根际中发现了最高的总铬浓度（204.6 mgkg-1）。棘皮动物。为了确定抗铬酸盐细菌的数量和百分比，对当地的微生物种群进行了不同浓度的Cr（VI）处理（以K2Cr2O7为40、100、300和1000 mM）。在培养基中的铬含量为1000 mM时，约25％的土壤细菌和45％的根际细菌均具有抗药性。在34个细菌分离物中，在24小时之内，仅嗜热脂肪芽孢杆菌12 ms，假单胞菌sp.12 bk3和粘粒沙雷氏菌7能还原50μMCr（VI）。使用延长的72 h孵育，他们能够减少添加到培养基中的500μMCr（VI）浓度。这些从铝土矿土壤中生长的植物的根际中分离出来的抗铬酸盐细菌具有很大的生物修复铬（VI）污染废物的潜力。

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