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首页> 外文期刊>African Journal of Biotechnology >Callus formation and organogenesis of tomato (Lycopersicon esculentum Mill, C.V. Omdurman) induced by thidiazuron
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Callus formation and organogenesis of tomato (Lycopersicon esculentum Mill, C.V. Omdurman) induced by thidiazuron

机译：噻二氮隆诱导的番茄的愈伤组织形成和器官发生（Lycopersicon esculentum Mill，C.V. Omdurman）

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In vitro?culture response was assessed in tomato (Lycopersicon esculentum?Mill. c. v. Omdurman) for optimum callus induction and plantlet regeneration. Callusinduction was achieved within seven to ten days directly on the cut surfaces?of both hypocotyls and cotyledon explants cultured on Murashige and Skoog (MS) basal medium supplemented with α-naphthalene acetic acid (NAA), 2,4-dichloro-phenoxyacetic acid (2,4-D), Thidiazuron (TDZ) and benzyl adenine (BA) alone or in different combinations, but not in hormone free-medium. The highest callusing index (5.3) was obtained on hypocotyls explants cultured on MS medium supplemented with NAA at 0.5 mg/l followed by an index of 5.2 obtained from the same explant by using 0.1 mg/l NAA in combination with BAP at 0.5 mg/l. However, for the cotyledon explants, the highest callusing index (4.7) was obtained on MS medium supplemented with NAA at either 2.0 or 3.0 mg/l. After 8 weeks of culture, organogenesis was observed only on the explants cultured on medium containing different concentrations of TDZ alone or in combination with BAP. The best shoot formation (93%) was obtained for cotyledon explant callus induced on MS medium containing TDZ in combination with BAP both at 0.5 mg/l. The highest number (6) of shoot per explant was obtained when cotyledon explant callus was sub cultured on MS medium supplemented with 3.0 mg/l TDZ. Plain half strength of MS was found to be the best rooting medium, however, addition of IAA at 1.0 mg/l and IBA at 2.0 mg/l were found essential to induce highest number of roots (22.1 ± 0.9) and longer roots (11.0 ± 0.3 cm), respectively. This protocol would be useful to create somaclonal variation and utilize transgenic approaches for varietal improvement?of tomato.
机译：在番茄（Lycopersicon esculentum？Mill。c。v。Omdurman）中评估了体外培养反应，以优化愈伤组织的诱导和苗的再生。直接在Murashige和Skoog（MS）基础培养基中添加辅以α-萘乙酸（NAA），2,4-二氯苯氧基乙酸（ 2,4-D），噻二唑酮（TDZ）和苄基腺嘌呤（BA）单独使用或以不同组合使用，但在无激素培养基中则不然。在补充有0.5 mg / l NAA的MS培养基上培养的下胚轴外植体获得最高的愈伤指数（5.3），然后通过使用0.1 mg / l NAA和BAP于0.5 mg / l的同种外植体获得的最高指数为5.2。 l。但是，对于子叶外植体，在补充有2.0或3.0 mg / l的NAA的MS培养基上可获得最高的愈伤组织指数（4.7）。培养8周后，仅在含有单独浓度的TDZ或与BAP组合的TDZ的培养基上培养的外植体上观察到器官发生。对于在含有TDZ和BAP的MS培养基（均为0.5 mg / l）上诱导的子叶外植体愈伤组织，可获得最佳的芽形成（93％）。当子叶外植体愈伤组织在补充了3.0 mg / l TDZ的MS培养基上进行亚培养时，获得最高数量（6）的外植体芽。已发现MS的普通半强度是最好的生根培养基，但是，发现添加1.0 mg / l的IAA和2.0 mg / l的IBA对于诱导最大数量的根（22.1±0.9）和更长的根（11.0）是必不可少的±0.3厘米）。该方案将有助于创建体细胞克隆变异并利用转基因方法改善番茄的品种。

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