A new protocol for extraction of C0t-1 DNA from rice

Chen Yan; Guan Li; Liu Hong; Li Gang; Qin Rui

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A new protocol for extraction of C0t-1 DNA from rice

机译：从水稻中提取C0t-1 DNA的新方案

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C0t-1?DNA, enriched for repetitive DNA sequences, has been proved to be valuable in the studies of plant species differentiation and genome evolution. A new protocol to steadily obtain the aimed range of DNA fragments has been developed by shearing the genomic DNA with the digest system containing DNase Ι and DNA polymerase Ι. Comparison through fluorescence?in situ?hybridization (FISH) -?Oryza sativa?L with labeled probe of?C0t-1?DNA isolated by the autoclave equipment and the new procedure was carried out. FISH result shows that the new procedure can be a feasible method to steadily isolate high-quality?C0t-1?DNA.

机译：富含C0t-1αDNA的重复DNA序列，已被证明在植物物种分化和基因组进化研究中具有重要价值。通过用含有DNase 1和DNA聚合酶1的消化系统剪切基因组DNA，已经开发出稳定获得目标DNA片段范围的新方案。通过荧光原位杂交（FISH）-水稻（Oryza sativa）L与高压釜设备分离的？C0t-1？DNA标记探针进行比较，并进行了新的程序。 FISH结果表明，该新方法是稳定分离高质量？C0t-1？DNA的可行方法。

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《African Journal of Biotechnology》 |2010年第28期|共4页
作者
Chen Yan; Guan Li; Liu Hong; Li Gang; Qin Rui;
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中图分类生物工程学（生物技术）;
关键词
C0t-1 DNADeoxyribonuclease ΙDNA polymerase Ιricerepetitive DNA sequence;

机译：C0t-1 DNA脱氧核糖核酸酶ΙDNA聚合酶竞争性DNA序列;

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3. Simple and Efficient Protocol for RNA and DNA extraction from Rice (Oryza sativa L.) for Downstream Applications [J] . Vibhuti M Jhala, Viralkumar B Mandaliya, Vrinda S Thaker International Research Journal of Biological Sciences . 2015,第2期

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A new protocol for extraction of C0t-1 DNA from rice

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