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首页> 外文期刊>African Journal of Biotechnology >Occurrence, isolation and DNA identification of Streptococcus thermophilus involved in Algerian traditional butter Smen
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Occurrence, isolation and DNA identification of Streptococcus thermophilus involved in Algerian traditional butter Smen

机译：阿尔及利亚传统黄油Smen中嗜热链球菌的发生，分离和DNA鉴定

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摘要

Streptococcus thermophilus?isolates from traditional butter ‘Smen’, a fermented product from cow’s and ewe’s milk in arid area was subjected to taxonomical investigations. The identification procedure included phenotypic approaches, molecular characterization by using genus polymerase chain reaction (PCR) amplifications for?sodA?gene encoding the manganese-dependant superoxide dismutase A, and species-specific primers from gene encoding glucose kinase(glcK), gene encoding DNA polymerase III?(dnaE) and gene encoding threonyl-tRNA synthetase?(thrS) housekeeping genes in order to distinguish among reference, and wild strains of?S. thermophilus?and for their differentiation from?Enterococcus?spp. A total of 12 strains were tested by DNA identification analysis and these indigenous isolates were unambiguously characterised by their housekeeping gene profiles. Finally, four genotypes were recognised. To our knowledge, this is the first report on the isolation and molecular characterization of?S. thermophilus?strains from fermented milk transformed into home-production butter provided from Southern West Algeria. These results are of particular interest as they favour the selection of future cultures starters as?S. thermophilus?from traditional dairy products. It is, therefore, of great importance to preserve the genetic pool of the wild strains.
机译：嗜热链球菌是从传统黄油“ Smen”中分离出来的，该黄油是在干旱地区从牛乳和母羊乳中发酵得到的产品，经过分类研究。鉴定程序包括表型方法，通过使用属聚合酶链反应（PCR）扩增编码依赖于锰的超氧化物歧化酶A的？sodA？基因的分子特征，以及来自编码葡萄糖激酶（glcK）的基因，编码DNA的基因的物种特异性引物为了区分参考系和野生系的？S，聚合酶III？（dnaE）和苏氨酸-tRNA合成酶？（thrS）管家基因编码的基因。嗜热菌及其与肠球菌的区别。通过DNA鉴定分析对总共12个菌株进行了测试，这些本土分离株的管家基因谱清晰地进行了表征。最后，识别出四种基因型。据我们所知，这是关于？S的分离和分子表征的第一份报告。发酵乳中的嗜热菌菌株转变为由阿尔及利亚南部提供的自产黄油。这些结果特别令人感兴趣，因为它们有利于选择未来的文化初学者。来自传统乳制品的嗜热菌。因此，保护​​野生株的遗传库非常重要。

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