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外文期刊>African Journal of Biotechnology
>Optimization of ultrasonic extraction of mycelial polysaccharides from Paecilomyces hepiali using response surface methodology and its antioxidant activity
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Optimization of ultrasonic extraction of mycelial polysaccharides from Paecilomyces hepiali using response surface methodology and its antioxidant activity
Ultrasonic technology was applied to extract mycelial polysaccharides fromPaecilomyces hepiali?and the process was optimized by response surface methodology. Antioxidant activity of polysaccharides was also investigated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (%DPPHsc).?Three independent variables?were ratio of water to raw material (x1), ultrasonic power (x2) and extraction time (x3). Statistical analysis indicated that x1, x2, x12, x32, x1x2, x1x3and x2x3?had significant effect on the yields. Besides, x2, x12?and x22?shaped the %DPPHsc of polysaccharides significantly.?Three dimensional?surface plots and contour?plots were drawn from the mathematical model. The optimal conditions for yield and %DPPHsc were as follows: condition (1) x1?= 125 mL/g, x2?= 300 W, x3?= 12 min, and condition (2) x1?= 125 mL/g and x2?= 500 W, x3?=?11 min, respectively. Under these conditions, the yield was 9.37%, and %DPPHsc was 45.34%. There existed good agreement between experimental and predicted values.
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机译：应用超声波技术从肝拟青霉中提取菌丝体多糖，并通过响应面法对其工艺进行了优化。还通过2,2-二苯基-1-吡啶并肼基（DPPH）自由基清除活性（％DPPHsc）研究了多糖的抗氧化活性。三个独立变量是水与原料的比（x1），超声功率（x2）和提取时间（x3）。统计分析表明，x1，x2，x12，x32，x1x2，x1x3和x2x3对产量有显着影响。此外，x2，x12和x22显着改变了多糖的％DPPHsc。从数学模型绘制了三维表面图和轮廓图。产率和％DPPHsc的最佳条件如下：条件（1）x1？= 125 mL / g，x2？= 300 W，x3？= 12分钟，条件（2）x1？= 125 mL / g和x2 λ＝ 500W，x3 ＝＝ 11分钟。在这些条件下，产率为9.37％，％DPPHsc为45.34％。实验值和预测值之间存在良好的一致性。
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