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首页> 外文期刊>African Journal of Biotechnology >Molecular characterization of HbEREBP2, a jasmonate-responsive transcription factor from Hevea brasiliensis Muell. Arg.
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Molecular characterization of HbEREBP2, a jasmonate-responsive transcription factor from Hevea brasiliensis Muell. Arg.

机译：HbEREBP2的分子表征，一种来自巴西橡胶树的茉莉酸酯响应转录因子。精氨酸

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摘要

Transcription factors of AP2/ERF superfamily are generally involved in defense responses of plants to biotic and abiotic stresses. Although,?defense proteins are present in abundance in laticifers of rubber tree, little is known about their transcriptional regulation. In this study, a full length cDNA, referred to asHbEREBP2?was characterized by means of bioinformatic analysis and quantitative real-time RT-PCR. The?HbEREBP2?was 786-bp in length and contained a 480-bp open reading frame (ORF) encoding a protein of 159 amino acid residues. Bioinformatic analysis showed that the deduced amino acid sequence of HbEREBP2 had a specific domain of AP2 superfamily and shared relative high identity with members of?CBF/DREB subfamily?from different plant species. Quantitative real-time RT-PCR revealed that methyl jasmonate was more effective than ethylene and rapidly than mechanical wounding on up-regulating?HbEREBP2expression. The results suggest that HbEREBP2 may be involved in the regulation of jasmonate-mediated defense responses in laticifers of rubber tree.
机译：AP2 / ERF超家族的转录因子通常参与植物对生物和非生物胁迫的防御反应。尽管防御蛋白大量存在于橡胶树的乳胶中，但对其转录调控知之甚少。在这项研究中，通过生物信息学分析和定量实时RT-PCR表征了全长cDNA，称为HbEREBP2α。 “ HbEREBP2”的长度为786bp，并含有一个480bp的开放阅读框（ORF），其编码159个氨基酸残基的蛋白质。生物信息学分析表明，推导的HbEREBP2氨基酸序列具有AP2超家族的特定结构域，并且与来自不同植物物种的“ CBF / DREB亚家族”的成员具有相对较高的同一性。实时定量RT-PCR显示，茉莉酸甲酯在上调？HbEREBP2表达上比乙烯更有效，并且比机械性损伤更快。结果表明，HbEREBP2可能参与了橡胶树胶束中茉莉酸介导的防御反应的调控。

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