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首页> 外文期刊>African Journal of Biotechnology >Hematological and enzymatic responses of Nile tilapia Oreochromis niloticus during short and long term sublethal exposure to zinc
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Hematological and enzymatic responses of Nile tilapia Oreochromis niloticus during short and long term sublethal exposure to zinc

机译：尼罗罗非鱼Oreochromis niloticus在短期和长期亚致死性锌暴露中的血液学和酶促反应

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Freshwater fish?Oreochromis niloticus?were exposed to?sublethal concentrations of zinc for short and long terms.?Responses of hematological parameters which include red blood cells count (RBC) and hemoglobin content (Hb) were investigated. Serumaspartate aminotransferase (AST)?and?alanine aminotransferase?(ALT)?activities werealso?determined.?Two hundred and forty fish (24.30 ± 2.85 g) were distributed randomly in twenty four glass aquaria (80 L) with stocking density of 10 fish?for?each. The aquaria were divided into two groups: the first group was exposed for one week to 2, 4 and 6 mg/L of ZnCl2?which represent 0.25, 0.50 and 0.75 of Zn LC50, respectively with three replicates for each concentration, in addition to 0.00 LC50?as?the?control; while the second group was exposed to the same concentrations of ZnCl2?with the same replicates for 4 weeks. The results of hematological parameters indicated that RBC and HB were significantly increased with increasing the concentration of ZnCl2sequentially for both short and long exposure periods. The enzymatic estimation showed that the activity of AST and ALT?also?significantly increased sequentially in zinc treated fish at both exposure periods. Results of the present study suggest thatsublethal concentrations of zinc affect the?hematological changes and?impaired liver functions. These?parameters can be useful in environmental biomonitoring of zinc contamination.
机译：淡水鱼或尼罗罗非鱼短期或长期接触亚致死浓度的锌。研究了血液学参数对红细胞计数（RBC）和血红蛋白含量（Hb）的响应。还确定了血清天门冬氨酸转氨酶（AST）和“丙氨酸转氨酶”（ALT）的活性。将240条鱼（24.30±2.85 g）随机分布在二十四个玻璃水族箱（80 L）中，放养密度为10条鱼为每个。将水族箱分为两组：第一组暴露于分别为0.25、0.50和0.75的Zn LC50的ZnCl22、2、4和6 mg / L一周，除了每种浓度外，还重复三遍0.00 LC50作为控制;而第二组则暴露于相同浓度的ZnCl2α中，并重复进行4周。血液学参数的结果表明，无论长期暴露时间长短，随着ZnCl2浓度的增加，RBC和HB均显着增加。酶促估计表明，在两个暴露时期，锌处理鱼的AST和ALT？活性也显着增加。本研究结果表明锌的致死浓度影响血液学变化和肝功能受损。这些参数可用于锌污染的环境生物监测。

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