Rubisco-bis-phosphate oxygenase (RuBP)- A potential housekeeping gene for qPCR assays in tea

Bornali Gohain; Tirthankar Bandyopadhyay; Priyadarshini Bhorali; Sangeeta Borchetia; Raju Bharalee; Sushmita Gupta; Niraj Agarwala; Neelakshi Bhattacharyya; Ranjit Singh; Prasenjit Bhagawati; Parveen Ahmed; M.C.Kalita; Sudripta Das

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Rubisco-bis-phosphate oxygenase (RuBP)- A potential housekeeping gene for qPCR assays in tea

机译：Rubisco-双磷酸加氧酶（RuBP）-茶中qPCR检测的潜在看家基因

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The present experiment is an effort to find a stable reference gene in?Camellia sinensisand?Camellia assamica?under different biotic and abiotic stresses.?This study evaluatethe variation in gene expression across tea leaf tissues in nine experiments. The suitability of?18S rRNA, 26S rRNA,?rubisco-bis-phosphatase?oxygenase?(RuBP) andCamellia?tubulin?(CaT)?as reference genes were validated by geNorm and BestKeeper programs. The finding reveals?18S?rRNA?and?RuBP?to be the most stably expressed housekeeping genes, the latter being the first report of its kind in tea. The finding paves the way for their application in accurate quantification of trait specific gene expression and other genomic studies in tea.

机译：本实验旨在在不同的生物和非生物胁迫下，在中国茶树和中国茶树中寻找稳定的参考基因。本研究在九个实验中评估了整个茶叶组织中基因表达的变化。 geNorm和BestKeeper程序验证了18S rRNA，26S rRNA，rubisco-bis-磷酸酶加氧酶（RuBP）和山茶花微管蛋白（CaT）作为参考基因的适用性。这一发现揭示了“ 18S” rRNA和“ RuBP”是最稳定表达的管家基因，后者是茶中此类基因的首次报道。这一发现为将其应用于对性状特异性基因表达的准确定量和茶中的其他基因组研究铺平了道路。

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《African Journal of Biotechnology》 |2012年第51期|共7页
作者
Bornali Gohain; Tirthankar Bandyopadhyay; Priyadarshini Bhorali; Sangeeta Borchetia; Raju Bharalee; Sushmita Gupta; Niraj Agarwala; Neelakshi Bhattacharyya; Ranjit Singh; Prasenjit Bhagawati; Parveen Ahmed; M.C.Kalita; Sudripta Das;
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中图分类生物工程学（生物技术）;
关键词
Camellia sinensisCamellia assamicaqPCRBestKeepergeNormhousekeeping gene;

机译：茶花山茶茶asPCR最佳保育植物保育基因;

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专利

1. Reversed‐phase ion‐pair Chromatographic separation of ribulose 1,5‐bisphosphate from 3‐phosphoglycerate and its application as a new enzyme assay for RuBP carboxylase/oxygenase [J] . Jakob Robert, Saenger Wolfram FEBS Letters . 1985,第1期

机译：反相离子对色谱法分离3-磷酸甘油酸中的核糖1,5-双磷酸酯及其在RuBP羧化酶/加氧酶的新酶分析中的应用
2. Biochemical and genetic analysis of an RuBP carboxylase/oxygenase‐deficient mutant and revertants of Chlamydomonas reinhardii [J] . Jordan D.B., Ogren W.L., Spreitzer R.J. FEBS Letters . 1982,第1期

机译：莱茵衣藻RuBP羧化酶/加氧酶缺陷型突变体和回复株的生化和遗传分析
3. Validation of Common Housekeeping Genes as Reference for qPCR Gene Expression Analysis During iPS Reprogramming Process [J] . Yulia Panina, Arno Germond, Shinji Masui, Scientific reports. . 2018,第1期

机译：验证iPS重编程过程中常见管家基因作为qPCR基因表达分析的参考
4. THE BALANCE BETWEEN CAPACITIES OF RuBP CARBOXYLATION AND RuBP REGENERATION: INTERSPECIFIC VARIATION IN RESPONSE. TO SEASONAL ENVIRONMENT [C] . Yusuke Onoda, Kouki Hikosaka, Tadaki Hirose International Congress of Photosynthesis . 2005

机译：RUBP羧化和RUBP再生能力之间的平衡：响应的间隙变化。到季节性环境
5. Plastic Biotransformation Technologies: Development of a Novel Environmental QPCR Assay for Polyethylene Terephthalate Hydrolase, and Isolation/Characterization of Polyethylene Degrading Fungi and Bacteria from Environmental Samples [D] . Wedin, Nelson Peter. 2020

机译：塑料生物转化技术：开发新型环境QPCR测定的聚对苯二甲酸乙二醇酯水解酶，以及来自环境样品的聚乙烯降解真菌和细菌的分离/表征
6. Validation of Common Housekeeping Genes as Reference for qPCR Gene Expression Analysis During iPS Reprogramming Process [O] . Yulia Panina, Arno Germond, Shinji Masui, -1

机译：验证iPS重编程过程中常见管家基因作为qPCR基因表达分析的参考
7. Reversed-phase ion-pair Chromatographic separation of ribulose 1,5-bisphosphate from 3-phosphoglycerate and its application as a new enzyme assay for RuBP carboxylase/oxygenase [O] . Jakob Robert, Saenger Wolfram 1985

机译：反相离子对色谱法从3-磷酸甘油酸酯中分离核果糖1,5-二磷酸核糖及其在RuBP羧化酶/加氧酶的新酶分析中的应用
8. Nature and alternate rates of the ribulose 1,5-bisphosphate (RuBP) carboxylase/oxygenase (Rubisco) oxygenation intermediate [R] . Harpel, M. R. , Chen, Y. R. , Hartman, F. C. 1995

机译：核酮糖1,5-二磷酸（RuBp）羧化酶/加氧酶（Rubisco）氧化中间体的性质和交替率

Rubisco-bis-phosphate oxygenase (RuBP)- A potential housekeeping gene for qPCR assays in tea

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