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首页> 外文期刊>African Journal of Biotechnology >Cloning of a vacuolar H+-pryophosphatase gene from emphemeral plant Olimarabidopsis pumila whose overexpression improve salt tolerance in tobacco
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Cloning of a vacuolar H+-pryophosphatase gene from emphemeral plant Olimarabidopsis pumila whose overexpression improve salt tolerance in tobacco

机译：临时植物短叶拟南芥的液泡H +-磷酸酶基因的克隆，其过表达提高了烟草的耐盐性

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Olimarabidopsis pumila is a close relative of the model plant Arabidopsis thaliana but, unlike A. thaliana, it is a salt-tolerant ephemeral plant that is widely distributed in semi-arid and semi-salinized regions of the Xinjiang region of China, thus providing an ideal candidate plant system for salt tolerance gene mining. The vacuolar H+-translocating inorganic pyrophosphatase (V-H+-PPase) is an electrogenic proton pump that play pivotal role in translocating protons into vacuoles in plant cells. A V-H+-PPase gene, OpVP, was isolated from O. pumila in this study. The OpVP cDNA has an open reading frame of 2 313 bp, encoding a polypeptide of 770 amino acid residues with an estimated molecular mass of 80.7 kDa. The OpVP shows high amino acid similarity with other Brassicaceae V-H+-PPase genes. Expression profiles under salt and drought treatment, abcisic acid (ABA), indole-3-aceticacid (IAA) and gibberellins (GA) induction were investigated, and the results reveal that the expression of OpVP was induced in leaves under treatment with salt, drought, ABA, IAA and GA3. Overexpression of the OpVP gene confers enhanced salt tolerance to the transformed tobacco. Transgenic tobacco grows well in the presence of 200 mM NaCl, while wild-type plants exhibit chlorosis and growth inhibition, even death. Compared with wild-type, transgenic plants accumulated more Na+ in leaves. Moreover, the leaves of transgenic plants retain higher chlorophyll content during salt stress. This study shows that OpVP is a potential gene for salt tolerance, and can be used in future for developing salt tolerant crops.
机译：拟南芥与拟南芥拟南芥的近缘种，但与拟南芥不同，它是一种耐盐的短暂植物，广泛分布于中国新疆地区的半干旱和半咸化地区，从而提供了一种耐盐基因挖掘的理想候选植物系统。液泡H +易位的无机焦磷酸酶（V-H + -PPase）是一种电质子泵，在质子转移到植物细胞的液泡中起关键作用。在这项研究中，从O. pumila中分离了一个V-H + -PPase基因OpVP。 OpVP cDNA具有2 313 bp的开放阅读框，编码770个氨基酸残基的多肽，估计分子量为80.7 kDa。 OpVP与其他十字花科V-H + -PPase基因具有高度的氨基酸相似性。研究了盐和干旱，脱落酸（ABA），吲哚-3-乙酸（IAA）和赤霉素（GA）诱导下的表达谱，结果表明，在盐，干旱处理下，OpVP的表达被诱导，ABA，IAA和GA3。 OpVP基因的过表达赋予转化烟草以更高的耐盐性。转基因烟草在200 mM NaCl存在下生长良好，而野生型植物则表现出萎黄病和生长抑制作用，甚至死亡。与野生型相比，转基因植物在叶片中积累了更多的Na +。此外，转基因植物的叶片在盐胁迫期间保留了较高的叶绿素含量。这项研究表明，OpVP是耐盐的潜在基因，将来可用于开发耐盐作物。

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