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首页> 外文期刊>African Journal of Biotechnology >Assessment of genetic diversity among cultivated Pearl millet (Pennisetum glaucum, Poaceae) accessions from Benin, West Africa
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Assessment of genetic diversity among cultivated Pearl millet (Pennisetum glaucum, Poaceae) accessions from Benin, West Africa

机译：西非贝宁栽培珍珠粟（Pennisetum glaucum，禾本科）种间遗传多样性的评估

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摘要

Simple sequence repeat (SSR) molecular markers were used for genetic diversity analysis and population structure of the cultivated Pearl millet in Benin, West Africa. In order to assess the level of genetic diversity, 14 polymorphic SSR markers were used to screen 114 accessions from different agro-ecological zones in Benin. SSR markers were found to reveal a total of 57 alleles with an average of 4.071 allele per locus. Genetic diversity index varied from 0.099 to 0.633 with an average of 0.405. The average observed heterozygosity was found to reach 0.425. The analysis of molecular variance showed no real differentiation between regions. Only 5% of genetic variation was observed between samples collected from north-eastern and north-western region. A high level of variation (95%) was observed among accessions. Moreover, both principal component analysis (PCA) and the dendrogram obtained from the genetic distance among accessions revealed the absence of any specific structuration of accessions from each region under study. Our results confirmed diversity among cultivated Pearl millet in Benin and such diversity is not clustering according to geographical patterns.
机译：简单序列重复（SSR）分子标记用于西非贝宁栽培珍珠小米的遗传多样性分析和种群结构。为了评估遗传多样性的水平，使用了14个多态性SSR标记来筛选来自贝宁不同农业生态区的114个种质。发现SSR标记揭示了总共57个等位基因，每个基因座平均4.071个等位基因。遗传多样性指数从0.099到0.633，平均为0.405。发现观察到的平均杂合度达到0.425。分子变异分析表明区域之间没有真正的区别。从东北和西北地区收集的样本之间仅观察到5％的遗传变异。在种质之间观察到高水平的变异（95％）。而且，主成分分析（PCA）和从种质间遗传距离获得的树状图均显示，每个研究区域的种质均没有任何特定的结构。我们的结果证实了贝宁栽培的珍珠粟之间的多样性，并且这种多样性并未根据地理格局进行聚类。

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