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首页> 外文期刊>African Journal of Biotechnology >Diversity and extracellular enzymes of endophytic fungi associated with Cymbidium aloifolium L.
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Diversity and extracellular enzymes of endophytic fungi associated with Cymbidium aloifolium L.

机译：与大花ym兰相关的内生真菌的多样性和细胞外酶。

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Cymbidium aloifolium is an epiphytic orchid used in treatment of many human ailments. The endophytic fungi associated with orchids are diverse and have potential to produce many bioactive compounds including extracellular enzymes. A total of 165 endophytic fungi representing 22 different fungal species were obtained from root, leaf and flowers of C. aloifolium. The colonization rate (CR) and isolation rate (IR) varied with different plant parts and was highest in root (CR = 40.6%, IR = 0.83) followed by leaf (CR = 32.12%, IR = 0.66) and flower (CR = 27.27%, IR = 0.56). The diversity of isolated endophytic fungi in root, leaf and flower was determined; Shannon-Wiener index (H’) was highest in root (H’ = 2.64) followed by leaf (H’ = 2.12) and flower (H’ = 1.5). Simpson diversity index (D’) was high in root (D’ = 0.93) with a maximum of 16 species, followed by leaf (D’= 0.88) with 9 species and flower (D’ = 0.78) with 5 species. Shannon evenness index (J’) was highest in leaf (J’ = 0.96) followed by root (J’= 0.95) and flower (J’ = 0.93). The endophytic fungi subjected for production of extracellular enzymes; 93% produced phosphatase, 80% cellulase, 70% amylase, 63.33% protease, 30% pectinase, 23.33% lipase and 10% laccase.
机译：大花ym兰是一种附生兰花，用于治疗许多人类疾病。与兰花相关的内生真菌种类繁多，具有产生包括细胞外酶在内的许多生物活性化合物的潜力。从C. aloifolium的根，叶和花中总共获得了代表22种不同真菌种类的165种内生真菌。移殖率（CR）和分离率（IR）因植物部位不同而异，根系最高（CR = 40.6％，IR = 0.83），其次是叶片（CR = 32.12％，IR = 0.66）和花朵（CR = 27.27％，IR = 0.56）。确定了分离的内生真菌在根，叶和花中的多样性;香农-维纳指数（H'）的根系最高（H'= 2.64），其次是叶片（H'= 2.12）和花朵（H'= 1.5）。辛普森多样性指数（D'）的根系最高（D'= 0.93），最多16种，其次是叶子（D'= 0.88）有9种，而花朵（D'= 0.78）有5种。香农均匀度指数（J’）在叶片中（J’= 0.96）最高，其次是根部（J’= 0.95）和花朵（J’= 0.93）。内生真菌产生细胞外酶; 93％的人产生了磷酸酶，80％的纤维素酶，70％的淀粉酶，63.33％的蛋白酶，30％的果胶酶，23.33％的脂肪酶和10％的漆酶。

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