首页> 外文期刊>African Journal of Biotechnology >Effectiveness of solid carriers on multiplication of bradyrhizobium mutant strain and nodulation of cowpea (Vigna unguiculata L)
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Effectiveness of solid carriers on multiplication of bradyrhizobium mutant strain and nodulation of cowpea (Vigna unguiculata L)

机译:固体载体对bra草根瘤菌突变株繁殖和of豆结瘤的有效性

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Effective nodulation of legume is usually solid carriers dependent. Peat, the most effective solid carrier is not readily available in Nigeria, hence, alternative solid carriers were evaluated with Bradyrhizobium strains using cowpea as a test crop. Mutant strains (USDA 3384 and USDA 3451) were evaluated in the laboratory using solid carriers and pot experiments. The survival (multiplication), infectivity and nodulating ability of the Bradyrhizobium mutant strains using cowpea as a test crop were assayed in peat, cowdung and composted maize cob. Mutant strains inoculated in the three solid carriers got to their peak of growth at 16th day of incubation. Peat significantly (p<0.05) increased the population of both USDA 3384 and USDA 3451 compared to cowdung and composted maize cob. USDA 3451 inoculated in cowdung significantly (p<0.05) increased multiplication of Bradyrhizobium mutant strain and nodulation of cowpea. Investigation shows it can be used as peat substitute when preparing inoculant. USDA 3384 inoculated in both composted maize cob and cowdung, respectively had consistent and significant positive effect on nodulation of cowpea and nodule dry weight. These results reveal their ability to subtitute peat. Hence, blind use of alternative solid carriers for inoculant preparations may hinder high infectivity and optimal nodulation which could facilitate positive effects of nitrogen fixation in low N tropical soil.
机译:豆科植物的有效结瘤通常取决于固体载体。泥炭是最有效的固体载体,在尼日利亚尚不容易获得,因此,使用Bra豆作为试验作物,利用缓生根瘤菌菌株对替代性固体载体进行了评估。在实验室中使用固体载体和盆栽实验评估了突变株(USDA 3384和USDA 3451)。以cow豆为试验作物,测定了在土壤泥炭,牛粪和玉米堆肥中的缓生根瘤菌突变菌株的存活(繁殖),感染力和结瘤能力。接种在三种固体载体中的突变菌株在培养第16天达到其生长高峰。与牛粪和堆肥玉米芯相比,泥炭显着(p <0.05)增加了USDA 3384和USDA 3451的种群。在牛粪中接种的USDA 3451显着(p <0.05)增强了根瘤菌突变菌株的繁殖和of豆的结瘤。调查显示,在制备孕育剂时,它可用作泥炭替代品。堆肥玉米芯和牛粪中分别接种的USDA 3384对cow豆的根瘤和根瘤干重均具有一致且显着的积极影响。这些结果揭示了它们替代泥炭的能力。因此,盲目使用替代固体载体来制备孕育剂可能会阻碍高感染力和最佳结瘤作用,从而促进低氮热带土壤固氮的积极作用。

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