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首页> 外文期刊>African Journal of Biotechnology >Potential applications of enzymes on the extraction of vitexin from dried Mas Cotek leaves
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Potential applications of enzymes on the extraction of vitexin from dried Mas Cotek leaves

机译：酶在干燥的Mas Cotek叶片中提取葡萄黄素的潜在应用

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摘要

Mas Cotek or Ficus deltoidea var. deltoidea leaves extracts contain various beneficial properties such as antidiabetic, antihypoglycemic and antioxidant activities, which can be enzymatically extracted in water. Vitexin is one of the antioxidants and remarkable compound of flavone C-glycosides found in Mas Cotek leaves. Cellulase is one of the essential enzymes applied to hydrolyze cellulose into fermentable sugars and therefore, this enzyme was used to extract vitexin in this study. Single parameter approach was used to determine factors influencing the enzymatic-mediated extraction. These factors were enzyme concentration, sample-to-water ratio, temperature, incubation time and agitation. The maximum yield of vitexin found was 0.547% under the optimum conditions: sample-to-water ratio 1:10 g/mL, enzyme concentration 0.4%, incubation time of 240 min, temperature of 50 ± 0.02°C and stirring speed of 200 rpm. The highest amount of reducing sugar obtained was 4.039 mg/mL with the use of 0.4% cellulose. A correlation was also observed between the yield of vitexin and reducing sugars.
机译：Mas Cotek或Ficus deltoidea var。三角洲树叶提取物具有多种有益特性，例如抗糖尿病，抗降血糖和抗氧化活性，可以在水中以酶促的方式提取。 Vitexin是在Mas Cotek叶片中发现的黄酮C-糖苷的抗氧化剂和非凡的化合物之一。纤维素酶是用于将纤维素水解为可发酵糖类的必需酶之一，因此，该酶在本研究中用于提取vitexin。使用单参数方法来确定影响酶促提取的因素。这些因素是酶浓度，样品与水的比例，温度，孵育时间和搅拌。在最佳条件下，发现的葡萄黄素的最大产率为0.547％：样品与水的比例为1:10 g / mL，酶浓度为0.4％，孵育时间为240分钟，温度为50±0.02°C，搅拌速度为200转速使用0.4％的纤维素，获得的最高还原糖量为4.039 mg / mL。在葡萄黄素和还原糖的产量之间也观察到相关性。

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