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首页> 外文期刊>African Journal of Biotechnology >Identification of proteins involved in excess boron stress in roots of carrot (Daucus carota L.) and role of niacin in the protein profiles
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Identification of proteins involved in excess boron stress in roots of carrot (Daucus carota L.) and role of niacin in the protein profiles

机译：胡萝卜根中与过量硼胁迫有关的蛋白质的鉴定以及烟酸在蛋白质谱中的作用

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摘要

Plants are constantly challenged by various biotic and abiotic stresses in nature.Boron toxicity have become one of the important abiotic stress factor for plants.Boron toxicity responses of plants is re?ected by alterations in protein expression level, activity, location and concentration. In this study, we identified the proteins which respond to the boron excess stress in?Daucus carota?root cells, and examined the effects of niacin (nicotinamide) on the protein profiles of boron excess. Protein profile changes in responce to boron and niacin were compared with?the?control using?sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS PAGE)?gel electrophoresis. Protein?bands of?interest were?excised from gel and digested by trypsin.?The trypsin-digested peptides were analyzed on nanoLC-ESI-MS/MS.?Six different proteins involved in plant defense system were identified in total [CR16 (Major allergen Dau c1), glutathion peroxidase, glyoxylase I, ?socitrate dehydrogenase (NADP-+), adenosyl homocysteinase, EDPG precursor)]. Our results clearly demonstrated the presence of boron and niacin in growth medium stimulated expression and synthesis of proteins role in plant defence mechanism.
机译：植物一直受到自然界中各种生物和非生物胁迫的挑战。硼的毒性已成为植物重要的非生物胁迫因素之一。植物的硼毒性反应通过蛋白质表达水平，活性，位置和浓度的变化来反映。在这项研究中，我们鉴定了能应对胡萝卜单链根细胞中过量硼胁迫的蛋白质，并研究了烟酸（烟酰胺）对过量硼的蛋白质谱的影响。用十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS PAGE）凝胶电泳将对硼和烟酸响应的蛋白质谱变化与对照进行比较。从凝胶上切下感兴趣的蛋白带并用胰蛋白酶消化。用胰蛋白酶消化的肽在nanoLC-ESI-MS / MS上进行分析。在植物防御系统中共鉴定出六种不同的蛋白质[CR16（主要变应原Dau c1），谷胱甘肽过氧化物酶，乙醛酸酶I，柠檬酸脱氢酶（NADP- +），腺苷高半胱氨酸酶，EDPG前体）]。我们的结果清楚地证明了生长培养基中硼和烟酸的存在刺激了蛋白质的表达和合成在植物防御机制中的作用。

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