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Evaluation of nutritional substrate and physical stress (gamma irradiation) in b-glucan productivity by mushroom (Pleurotus ostreatus)

机译:蘑菇(平菇)对β-葡聚糖生产率的营养底物和身体压力(γ射线)的评估

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-Glucans present in mushrooms are bioactive polysaccharides that have many pharmacological (immuno-modulatory, antitumoral, hepatoprotector, anti- inflammatory and antioxidant) activities.?The?-glucans?productivity of the mushroom depends upon the growing conditions, nutritional substrates, biotic and abiotic stress factors that result in an enzymes over-expression. The study shows the potential response of glucan production by fungi grown on newly designed?animal plant crude (hydrolyzate) extract broth (APCEB) substrate (animal-, plant-waste crude extract broth fortified with 1g KH2PO4?l-1) and gamma irradiation.?On?application of physical stress factors (gamma irradiation, 1 to 6 kGy), 1 kGy gamma irradiation doses resulted in an increase in: protein (37.5%), carbohydrates (32.15%), glucans (12.0%) and growth (dry mass, 14.8%); these were due to the enhancement of low doses (1.0 kGy) of gamma irradiation upon growth and metabolism that was synergized by the radioprotective components (cysteine) that prevailed in the animal extract. This substrate supported the growth and glucan production of?Pleurotus ostreatus?than bySchizophyllum?commune?and?Saccharomyces?cerevisae. There are suggested procedures to assure the percentage of glucan purity and entity. The APCEB substrate is a general basic formula in which the animal and plant remains can be replaced by many others. The yield of this media economizes the glucan production.
机译:-蘑菇中存在的葡聚糖是具有多种药理(免疫调节,抗肿瘤,保肝,抗炎和抗氧化剂)活性的生物活性多糖。蘑菇的“-葡聚糖”生产力取决于生长条件,营养底物,生物和非生物应激因素导致酶的过度表达。研究表明,在新设计的动物植物粗(水解)提取物肉汤(APCEB)底物(动物,植物废物粗提取物肉汤中加入1g KH2PO4?l-1强化)和γ射线照射下生长的真菌对葡聚糖产生的潜在响应在施加物理压力因子(γ辐射,1至6 kGy),1 kGyγ辐射剂量后,蛋白质(37.5%),碳水化合物(32.15%),葡聚糖(12.0%)和生长(干重14.8%);这些是由于低剂量(1.0 kGy)的γ射线在生长和代谢时增强,而γ射线通过动物提取物中普遍存在的辐射防护成分(半胱氨酸)而协同作用。该底物支持平菇的生长和葡聚糖的产生,而不是通过Schizophyllum?commun?和?Saccharomyces?cerevisae。有建议的程序可确保葡聚糖纯度和实体百分比。 APCEB底物是一种通用的基本配方,其中的动植物残骸可以被许多其他植物替代。这种培养基的产量节省了葡聚糖的生产。

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