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首页> 外文期刊>African Journal of Biotechnology >Production and partial purification of extracellular tannase by Klebsiella pneumoniae MTCC 7162 isolated from tannery effluent
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Production and partial purification of extracellular tannase by Klebsiella pneumoniae MTCC 7162 isolated from tannery effluent

机译：从制革厂废水中分离出的肺炎克雷伯菌MTCC 7162生产并部分纯化胞外鞣酸酶

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摘要

Diverse bacteria belonging to various taxa were isolated from tannery effluent of leather industries located at Ranipet, India and were screened for the production of extracellular tannase. One of the strains identified as?Klebsiella pneumoniae?MTCC 7162 was found to produce tannase (3.4 U/ ml) at pH 6.0, 37°C and 100 rpm. Use of individual carbon and inorganic nitrogen sources resulted in lower tannase production. However, a combination of urea and corn steep liquor extract yielded marginal increase in tannase production (3.9 U/ ml). End-product repression was also studied with inclusion of gallic acid to the growth medium. Enrichment with various additives of metal ions and detergents resulted in inhibition of tannase production. The enzyme was partially purified using ammonium sulfate precipitation followed by the use of DEAE-cellulose. SDS-PAGE analysis indicated that the molecular weight of the protein to be 46.5 kDa. The enzyme was found to be active in a wide range of pH and temperature with an optimal activity at pH 5.5 and 40°C.
机译：从位于印度拉尼佩特（Ranipet）的皮革工业的制革厂废水中分离出属于各种分类单元的多种细菌，并对其进行细胞外鞣酸酶生产进行了筛选。发现一种被鉴定为“肺炎克雷伯氏菌” MTCC 7162的菌株在pH 6.0、37℃和100rpm下产生鞣酸酶（3.4U / ml）。使用单独的碳源和无机氮源会降低鞣酸酶的产量。但是，尿素和玉米浆提取物的组合在鞣酸酶生产中产生了少量增加（3.9 U / ml）。还研究了在生长培养基中包含没食子酸的最终产物抑制作用。富含各种金属离子和去污剂的添加剂导致抑制鞣酸酶的产生。使用硫酸铵沉淀，然后使用DEAE-纤维素，部分纯化该酶。 SDS-PAGE分析表明该蛋白质的分子量为46.5kDa。发现该酶在很宽的pH和温度范围内均具有活性，在pH 5.5和40°C下具有最佳活性。

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