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首页> 外文期刊>African Journal of Biotechnology >Application of Lentikats Biotechnology for removal of nitrates from ion-exchange brines: Implications for adaptation of encapsulated denitrifiers
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Application of Lentikats Biotechnology for removal of nitrates from ion-exchange brines: Implications for adaptation of encapsulated denitrifiers

机译：Lentikats生物技术在离子交换盐水中去除硝酸盐的应用：适应封装的反硝化剂的意义

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摘要

Denitrifying Bacterium?Paracoccus denitrificans?encapsulated in polyvinyl alcohol matrix (so called Lentikats Biocatalyst; LB) was applied for the removal of nitrates from simulated ion-exchange brines (12.14 g.L-1?Cl-, 1.35 g.L-1?SO42-, and 2.26 g.L-1N-NO3-). The effect of brines dilution on the denitrification activity of fresh Lentikats Biocatalysts was tested simultaneously for four dilutions (100% = non-diluted, 80% = 4:1 brine:water, 60 and 40%) in four sequent repetitions per dilution without intermediate cultivation. In the first set, the denitrification of 100 and 80% brine was severely inhibited, while the denitrification activity in 60 and 40% brines was comparable to activity in non-inhibiting denitrification medium. The denitrification activity in 60 and 40% brines declined in later repetitions due to a lack of nutrients, while it increased in 80 and 100% brines before it declined as well. Such results are in contradiction to previous observations based on correlation analyses of data from longer experimental period. The detailed comparison implicates the adaptation of the encapsulated denitrification bacteria to higher salinity and emphasizes its importance for achievement of high denitrification activities of Lentikats Biocatalyst in industrial-scale applications even in non-diluted brines.
机译：包裹在聚乙烯醇基体中的反硝化细菌？反硝化杆菌（称为Lentikats生物催化剂; LB）用于从模拟离子交换盐水（12.14 gL-1？Cl-，1.35 gL-1？SO42-和2.26 gL-1N-NO3-）。同时测试了盐水稀释对新鲜Lentikats生物催化剂反硝化活性的影响，同时进行了四次稀释（100％=未稀释，80％= 4：1盐水：水，分别为60％和40％），每次稀释重复四次，没有中间体培养。在第一组中，100％和80％盐水的反硝化作用受到严重抑制，而60％和40％盐水中的反硝化活性可与非抑制性反硝化介质相媲美。由于缺乏养分，后来在60％和40％盐水中的反硝化活性下降，而在80％和100％盐水中反硝化活性则在下降之前增加。这样的结果与以前基于较长实验数据的相关分析得出的结论相矛盾。详细的比较暗示了封装的反硝化细菌对更高盐度的适应性，并强调了其对于实现Lentikats生物催化剂在工业规模应用中甚至在未稀释盐水中实现高反硝化活性的重要性。

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