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A study on nitrogen removal efficiency of Pseudomonas stutzeri strains isolated from an anaerobic/anoxic/oxic wastewater treatment process

机译:厌氧/缺氧/有氧废水处理工艺分离的斯氏假单胞菌菌株脱氮效率的研究

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In order to improve the?nitrogen removal?efficiency?in?an anaerobic/anoxic/oxic treatment plant, a strain with high nitrification and denitrification capability was isolated from a specific anaerobic/anoxic/oxic treatment process. The characteristics of isolate were experimentally analyzed. By using the nitrogen balance method, the total nitrogen loss was calculated to be 40.1% (w/w) when the carbon source was citric acid with a C/N ratio of 5. Meanwhile, the isolated strain was identified by 16S rDNA to be a?Pseudomonas stutzeri?with a similarity of 99%. Varying the initial TN, the C/N, the pH value and the ambient temperature in the reaction system, the efficiency of nitrogen removal was studied. The results showed that the highest efficiency occurred when the C/N was 12, the pH value was 7 and the temperature was 32°C. The results were also compared to the practically monitoring data coming with a good agreement. Consequently, it is viable to improve the?nitrogen removal?efficiency by varying the reaction conditions.
机译:为了提高厌氧/缺氧/氧化处理厂的“除氮效率”,从特定的厌氧/缺氧/氧化处理工艺中分离出了具有高硝化和反硝化能力的菌株。对分离物的特性进行了实验分析。通过使用氮平衡法,当碳源为柠檬酸且C / N比为5时,总氮损失计算为40.1%(w / w)。同时,通过16S rDNA鉴定出分离的菌株为斯氏假单胞菌的相似性为99%。通过改变反应体系中的起始总氮,碳氮比,pH值和环境温度,研究了脱氮效率。结果表明,当C / N为12,pH值为7和温度为32°C时,效率最高。还将结果与实际达成一致的监测数据进行了比较。因此,通过改变反应条件来提高脱氮效率是可行的。

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