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首页> 外文期刊>African Journal of Biotechnology >Using molecular techniques for rapid detection of Salmonella serovars in frozen chicken and chicken products collected from Riyadh, Saudi Arabia
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Using molecular techniques for rapid detection of Salmonella serovars in frozen chicken and chicken products collected from Riyadh, Saudi Arabia

机译：使用分子技术快速检测从沙特阿拉伯利雅得收集的冷冻鸡肉和鸡肉产品中的沙门氏菌

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The current study was aimed to investigate the incidence of different?Salmonellaserovars in chicken products either from local or imported source. A total of 152 samples of chicken and chicken products were collected from different retail establishment markets in Riyadh, KSA including 38 local whole frozen chickens, 62 imported whole frozen chickens, 22 whole poultry eggs and 30 local chicken cuts samples and examined by standard microbiological techniques (SMT).?Salmonellaisolation revealed a total percentage of 5.92%; chicken cuts revealed a high incidence among the examined samples (10%), followed by local frozen chickens and imported frozen chicken samples with incidence of 7.89 and 4.83%, respectively. For this experiment, the whole chicken eggs were negative forSalmonella?species by SMT.?Salmonella enteritidis?was dominating among the recovered?Salmonella?serovars, followed by?Salmonella typhimurium, while only two strains of?Salmonella agona?and?Salmonella newport?were isolated. The PCR assay combined with Rappaport- Vassiliadis (RV) selective broth (PCR-RV) for the detection of?Salmonella?species in the collected field samples revealed the same positive samples directly from the imported frozen chickens and whole chicken eggs which gave negative results by SMT. Thus PCR-RV technique is rapid, time saving and applicable to detect?Salmonella?serovars directly from chicken samples.
机译：当前的研究旨在调查来自本地或进口来源的鸡肉产品中不同沙门氏菌的发生率。从KSA利雅得不同零售市场收集了152个鸡肉和鸡肉产品样本，包括38份本地全冻鸡，62份进口全冻鸡，22份家禽蛋和30份本地鸡块样本，并通过标准微生物技术进行了检查（SMT）。沙门氏菌分离显示总百分比为5.92％;鸡肉切块显示被检样品中的发病率很高（10％），其次是本地冷冻鸡和进口冷冻鸡样品，分别为7.89％和4.83％。对于该实验，通过SMT，整个鸡蛋对沙门氏菌属均为阴性。在回收的沙门氏菌血清型中，肠炎沙门氏菌占主导地位，其次是鼠伤寒沙门氏菌，而沙门氏菌和新港沙门氏菌只有两个菌株。被隔离了。 PCR检测方法与Rappaport-Vassiliadis（RV）选择性肉汤（PCR-RV）结合使用，用于检测采集的田间样品中的沙门氏菌种类，结果直接从进口的冷冻鸡和整只鸡卵中得到了相同的阳性样品，但结果均为阴性通过SMT。因此，PCR-RV技术快速，省时，可直接从鸡肉样品中检测沙门氏菌血清型。

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