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首页> 外文期刊>African Journal of Biotechnology >Mercury chloride-induced oxidative stress in human erythrocytes and the effect of vitamins C and E in vitro
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Mercury chloride-induced oxidative stress in human erythrocytes and the effect of vitamins C and E in vitro

机译：氯化汞诱导的人红细胞氧化应激及维生素C和E的体外作用

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Mercury can exist in the environment as metal, as monovalent and divalent salts and as organomercurials, one of the most important of which is mercuric chloride (HgCl2). It has been shown to induce oxidative stress in erythrocytes through the generation of free radicals and alteration of the cellular antioxidant defense system. The effect of simultaneous pretreatment with vitamins C and E on the toxicity of HgCl2?in human erythrocytes was evaluated. We examined the effect of several different doses of HgCl2?(1.052, 5.262, 10.524 μM), or HgCl2?in combination with vitamin C (VC; 10 μM) and vitamin E (VE; 30 μM), on the levels of malondialdehyde (MDA) and superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities in human erythrocytes?in vitro. Erythrocytes were incubated under various treatment conditions (HgCl2?alone, vitamins alone, or HgCl2?plus vitamin) at 37°C for 60 min and the levels of MDA and SOD, CAT and GPx activities, were determined. Treatment with HgCl2?alone increased the levels of MDA and decreased SOD, CAT and GPx activities in erythrocytes (P < 0.05). VC and VE-pretreated erythrocytes showed a significant protection aganist the cytotoxic effects induced by HgCl2?on the studied parameters. There were no statistical differences among VC+VE-treated erythrocytes, as compared to non-treated control cells. These results indicated that the presence of vitamins at concentrations that are similar to the levels found in plasma could be able to ameliorate HgCl2-induced oxidative stress by decreasing lipid peroxidation and altering antioxidant defense system in erythrocytes.
机译：汞可以金属，一价和二价盐以及有机汞的形式存在于环境中，其中最重要的一种是氯化汞（HgCl2）。它已经显示出通过自由基的产生和细胞抗氧化剂防御系统的改变来诱导红细胞中的氧化应激。评估了同时用维生素C和E预处理对HgCl2？对人红细胞毒性的影响。我们检查了几种不同剂量的HgCl2？（1.052、5.262、10.524μM）或HgCl2？与维生素C（VC; 10μM）和维生素E（VE; 30μM）组合对丙二醛（ MDA）和超氧化物歧化酶（SOD），过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）在人红细胞中的体外活性。将红细胞在各种处理条件下（单独的HgCl2 ?、单独的维生素或HgCl2？加维生素）在37°C下孵育60分钟，并测定MDA和SOD，CAT和GPx活性的水平。单独使用HgCl2？进行治疗会增加红细胞中的MDA水平，并降低SOD，CAT和GPx活性（P <0.05）。 VC和VE预处理的红细胞对HgCl2？对所研究参数的细胞毒性作用具有显着的保护作用。与未经处理的对照细胞相比，经VC + VE处理的红细胞之间无统计学差异。这些结果表明，与血浆中浓度相似的维生素存在可以通过减少脂质过氧化作用和改变红细胞的抗氧化防御系统来缓解HgCl2诱导的氧化应激。

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