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Analysis of three polymorphisms in Bidayuh ethnic of Sarawak population: A report from Malaysia

机译:砂拉越人口的比达友族三个多态性分析:马来西亚的一份报告

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Insertion/deletion polymorphism of YAP (DYS287), M96 and M120 polymorphisms in Bidayuh ethnic populations of Sarawak, Malaysia were analyzed in this?study. Genomic DNA was extracted from 180 buccal samples and amplified by Hot-Start PCR method. The amplified PCR products were separated by using 2% agarose gel electrophoresis. 26 out of 180 samples (14.44%) have shown insertion (YAP+) polymorphism, while the rest of the samples (85.56%) have shown deletion (YAP-) polymorphism. M96 derived and ancestral allele reveal 102 (56.67%) and 78 (43.33%), respectively and 96 (53.33%), while for M120 derived allele and 84 (46.67%) for M120 ancestral allele. This?study has provided the information about the distribution of YAP, M96 and M120 polymorphisms in Bidayuh ethnic population of Sarawak. This?study has to be continued with more samples to determine the YAP polymorphism and also the biallelic markers in Bidayuh population.?Keywords:?Y-chromosome, YAP, Sarawak.
机译:本研究分析了马来西亚沙捞越州比达友族人群中YAP(DYS287),M96和M120多态性的插入/缺失多态性。从180个颊样本中提取基因组DNA,并通过热启动PCR方法进行扩增。通过使用2%琼脂糖凝胶电泳分离扩增的PCR产物。 180个样本中有26个(14.44%)显示出插入(YAP +)多态性,而其余样本(85.56%)显示出缺失(YAP-)多态性。 M96衍生和祖先等位基因分别显示102(56.67%)和78(43.33%)和96(53.33%),而M120衍生等位基因和M120祖先等位基因显示84(46.67%)。这项研究提供了有关砂拉越比达尤族人口中YAP,M96和M120多态性分布的信息。该研究必须继续进行,以测定YAP多态性以及Bidayuh人群中的双等位基因标记。关键字:Y染色体,YAP,砂拉越。

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