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首页> 外文期刊>African Journal of Biotechnology >In vitro shoot regeneration from preconditioned explants of chickpea (Cicer arietinum L.) cv. Gokce
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In vitro shoot regeneration from preconditioned explants of chickpea (Cicer arietinum L.) cv. Gokce

机译：鹰嘴豆（Cicer arietinum L.）cv的预处理外植体的体外芽再生。戈切斯

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摘要

The present study reports the successful shoot regeneration of preconditioned mature embryo and embryonic axis explants of chickpea cv. Gokce. Explants were preconditioned with 10 mgl benzylaminopurine (BA) for 7 days followed by culture on Murashige and Skoog (MS) medium containing 0.25, 0.50, 1.00 and 2.00 mg/l BA with or without 0.25 mg/l naphthalene acetic acid (NAA) supplemented with 4?mg/l activated?charcoal and 1 mg/l polyvinylpyrrolidon (PVP). Shoot regeneration was recorded on all explants. Maximum number of (14.75) shoots per explants on mature embryo were recorded on MS medium containing 2.0 mg/l BA with 0.25 mg/l NAA. Whereas, 16.83 shoots per explants were recorded on MS medium containing 2.0 mg/l BA. Presence of NAA in the culture medium decreased the mean shoot length of both explants compared to medium devoid of 0.25 mg/l NAA. Regenerated shoots were rooted on MS medium containing 1.0 mg/l indole-butyric acid (IBA) after 4 weeks of culture. Rooted plantlets were transferred to pots for acclimatization under green house conditions.
机译：本研究报道了鹰嘴豆cv预处理的成熟胚和胚轴外植体的芽再生成功。 Gokce。将外植体用10 mgl苄基氨基嘌呤（BA）预处理7天，然后在含有0.25、0.50、1.00和2.00 mg / l BA的Murashige和Skoog（MS）培养基上培养，添加或不添加0.25 mg / l萘乙酸（NAA）加入4？mg / l的活性炭和1 mg / l的聚乙烯基吡咯烷酮（PVP）。记录所有外植体的芽再生。在含有2.0 mg / l BA和0.25 mg / l NAA的MS培养基上，记录了成熟胚上每个外植体的最大（14.75）个芽。而在含有2.0 mg / l BA的MS培养基上，每外植体记录了16.83个芽。与不含0.25 mg / l NAA的培养基相比，培养基中NAA的存在降低了两种外植体的平均芽长。培养4周后，将再生的芽生根于含有1.0mg / l吲哚丁酸（IBA）的MS培养基上。将生根的小植株转移到盆中以在温室条件下适应环境。

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